Prophage-Related Gene VpaChn25_0724 Contributes to Cell Membrane Integrity and Growth of Vibrio parahaemolyticus CHN25

• Published in: Frontiers in cellular and infection microbiology Vol. 10; p. 595709
• Main Authors: Yang, Lianzhi, Wang, Yaping, Yu, Pan, Ren, Shunlin, Zhu, Zhuoying, Jin, Yinzhe, Yan, Jizhou, Peng, Xu, Chen, Lanming
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 09.12.2020
• Subjects: Bacterial Proteins - genetics; Cell Membrane; Cellular and Infection Microbiology; Ecosystem; gene knockout; Humans; prophage; Prophages - genetics; secretome; Transcriptome; Vibrio parahaemolyticus; Vibrio parahaemolyticus - genetics; virulence; Vibrio parahaemolyticus; gene knockout; prophage; virulence; secretome; transcriptome
• ISSN: 2235-2988; 2235-2988
• DOI: 10.3389/fcimb.2020.595709

is a leading seafood-borne pathogen that can cause acute gastroenteritis and even death in humans. In aquatic ecosystems, phages constantly transform bacterial communities by horizontal gene transfer. Nevertheless, biological functions of prophage-related genes in remain to be fully unveiled. Herein, for the first time, we studied one such gene encoding an unknown hypothetical protein in CHN25. This gene deletion mutant Δ was constructed by homologous recombination, and its complementary mutant Δ -com was also obtained. The Δ mutant exhibited a sever defect in growth and swimming motility particularly at lower temperatures. Biofilm formation and cytotoxicity capacity of CHN25 was significantly lowered in the absence of . Comparative secretomic analysis revealed an increase in extracellular proteins of Δ , which likely resulted from its damaged cell membrane. Comparison of transcriptome data showed twelve significantly altered metabolic pathways in Δ , suggesting inactive transport and utilization of carbon sources, repressed energy production and membrane biogenesis in . Comparative transcriptomic analysis also revealed several remarkably down-regulated key regulators in bacterial gene regulatory networks linked to the observed phenotypic variations. Overall, the results here facilitate better understanding of biological significance of prophage-related genes remaining in .