Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum

• Published in: The Journal of biological chemistry Vol. 261; no. 28; pp. 12942 - 12947
• Main Authors: Shuber, A P, Orr, E C, Recny, M A, Schendel, P F, May, H D, Schauer, N L, Ferry, J G
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 05.10.1986; American Society for Biochemistry and Molecular Biology
• Subjects: Aldehyde Oxidoreductases - genetics; Amino Acid Sequence; Antibodies; Bacteriology; Base Sequence; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Cloning, Molecular; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Escherichia coli - genetics; Euryarchaeota - enzymology; Euryarchaeota - genetics; Formate Dehydrogenases - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Genetics; Macromolecular Substances; Microbiology; Mission oriented research; Gene; Archaeobacteria; Methanobacterium; Methanobacteriaceae; Bacteria; Primary structure; Formate dehydrogenase; Molecular cloning; Gene expression; Methanobacteriales
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(18)69253-1

The genes for the two subunits of the formate dehydrogenase from Methanobacterium formicicum were cloned and their sequences determined. When expressed in Escherichia coli, two proteins were produced which had the appropriate mobility on an SDS gel for the two subunits of formate dehydrogenase and cross-reacted with antibodies raised to purified formate dehydrogenase. The genes for the two formate dehydrogenase subunits overlap by 1 base pair and are preceded by DNA sequences similar to both eubacterial and archaebacterial promoters and ribosome-binding sites. The amino acid sequences deduced from the DNA sequence were analyzed, and the arrangement of putative iron-sulfur centers is discussed.