Designing and Constructing a Novel Artificial Pathway for Malonic Acid Production Biologically

Malonic acid is used as a common component of many products and processes in the pharmaceutical and cosmetic industries. Here, we designed a novel artificial synthetic pathway of malonic acid, in which oxaloacetate, an intermediate of cytoplasmic reductive tricarboxylic acid (rTCA) pathway, is conve...

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Published inFrontiers in bioengineering and biotechnology Vol. 9; p. 820507
Main Authors Gu, Shuying, Zhao, Zhen, Yao, Yonghong, Li, Jingen, Tian, Chaoguang
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 19.01.2022
Subjects
a-keto decarboxylase
Bioengineering and Biotechnology
malonic acid
metabolic engineering
Myceliophthora thermophila
oxaloacetic acid
oxaloacetic acid
a-keto decarboxylase
metabolic engineering
Myceliophthora thermophila
malonic acid
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Summary:Malonic acid is used as a common component of many products and processes in the pharmaceutical and cosmetic industries. Here, we designed a novel artificial synthetic pathway of malonic acid, in which oxaloacetate, an intermediate of cytoplasmic reductive tricarboxylic acid (rTCA) pathway, is converted to malonic semialdehyde and then to malonic acid, sequentially catalyzed by -keto decarboxylase and malonic semialdehyde dehydrogenase. After the systematic screening, we discovered the enzyme oxaloacetate decarboxylase Mdc, catalyzing the first step of the artificially designed pathway . Then, this synthetic pathway was functionally constructed in cellulolytic thermophilic fungus . After enhancement of glucose uptake, the titer of malonic acid achieved 42.5 mg/L. This study presents a novel biological pathway for producing malonic acid from renewable resources in the future.
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Reviewed by: Zheng-Jun Li, Beijing University of Chemical Technology, China
Edited by: Tao Chen, Tianjin University, China
This article was submitted to Synthetic Biology, a section of the journal Frontiers in Bioengineering and Biotechnology
Jiong Hong, University of Science and Technology of China, China
Guang Zhao, Shandong University, China
ISSN:2296-4185
2296-4185
DOI:10.3389/fbioe.2021.820507