Monoclonal antibody to the rat glucocorticoid receptor. Relationship between the immunoreactive and DNA-binding domain

• Published in: The Journal of biological chemistry Vol. 260; no. 21; pp. 11805 - 11810
• Main Authors: Eisen, L P, Reichman, M E, Thompson, E B, Gametchu, B, Harrison, R W, Eisen, H J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 25.09.1985; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Antibodies, immunoglobulins; Antibodies, Monoclonal - immunology; Binding Sites; Biological and medical sciences; Dexamethasone - analogs & derivatives; Dexamethasone - metabolism; DNA - metabolism; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Molecular immunology; Molecular Weight; Monoclonal antibodies; Peptide Fragments - analysis; Rats; Receptors, Glucocorticoid - immunology; Receptors, Glucocorticoid - isolation & purification; Receptors, Glucocorticoid - metabolism; Triamcinolone Acetonide - metabolism; Tritium; Vertebrata; Mammalia; Rat; Steroid hormone; Animal; DNA; Rodentia; Molecular interaction; Monoclonal antibody; Glucocorticoid; Structure function relationship; Hormonal receptor

The region of the glucocorticoid receptor that reacted with a monoclonal antibody (BUGR-1) was identified. In order to identify the immunoreactive region, the rat liver glucocorticoid receptor was subjected to limited proteolysis; immunoreactive fragments were identified by Western blotting. The mon...