Monoclonal antibody to the rat glucocorticoid receptor. Relationship between the immunoreactive and DNA-binding domain

• Published in: The Journal of biological chemistry Vol. 260; no. 21; pp. 11805 - 11810
• Main Authors: Eisen, L P, Reichman, M E, Thompson, E B, Gametchu, B, Harrison, R W, Eisen, H J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 25.09.1985; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Antibodies, immunoglobulins; Antibodies, Monoclonal - immunology; Binding Sites; Biological and medical sciences; Dexamethasone - analogs & derivatives; Dexamethasone - metabolism; DNA - metabolism; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Molecular immunology; Molecular Weight; Monoclonal antibodies; Peptide Fragments - analysis; Rats; Receptors, Glucocorticoid - immunology; Receptors, Glucocorticoid - isolation & purification; Receptors, Glucocorticoid - metabolism; Triamcinolone Acetonide - metabolism; Tritium; Vertebrata; Mammalia; Rat; Steroid hormone; Animal; DNA; Rodentia; Molecular interaction; Monoclonal antibody; Glucocorticoid; Structure function relationship; Hormonal receptor
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(17)39102-0

The region of the glucocorticoid receptor that reacted with a monoclonal antibody (BUGR-1) was identified. In order to identify the immunoreactive region, the rat liver glucocorticoid receptor was subjected to limited proteolysis; immunoreactive fragments were identified by Western blotting. The monoclonal antibody reacted with both the undigested Mr approximately 97,000 receptor subunit and a Mr approximately 45,000 fragment containing the steroid-binding and DNA-binding domains. Digestion by trypsin also produced two steroid-binding fragments of Mr approximately 27,000 and 31,000 which did not react with the antibody and an immunoreactive Mr approximately 16,000 fragment. This Mr approximately 16,000 fragment was shown to bind to DNA-cellulose, indicating that it contained a DNA-binding domain of the receptor. The undigested receptor must have steroid associated with it to undergo activation to a DNA-binding form. However, the Mr approximately 16,000 immunoreactive fragment binds to DNA-cellulose even if it is obtained by digestion of the steroid-free holoreceptor which does not itself bind to DNA.