Evaluation of Epigenetic Age Based on DNA Methylation Analysis of Several CpG Sites in Ukrainian Population

• Published in: Frontiers in genetics Vol. 12; p. 772298
• Main Authors: Kuzub, N, Smialkovska, V, Momot, V, Moseiko, V, Lushchak, O, Koliada, A
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 06.01.2022
• Subjects: aging; DNA methylation; epigenetic age; epigenetics clock; Genetics; pyrosequencing; DNA methylation; pyrosequencing; epigenetics clock; aging; epigenetic age
• ISSN: 1664-8021; 1664-8021
• DOI: 10.3389/fgene.2021.772298

Epigenetic clocks are the models, which use CpG methylation levels for the age prediction of an organism. Although there were several epigenetic clocks developed there is a demand for development and evaluation of the relatively accurate and sensitive epigenetic clocks that can be used for routine research purposes. In this study, we evaluated two epigenetic clock models based on the 4 CpG sites and 2 CpG sites in the human genome using the pyrosequencing method for their methylation level estimation. The study sample included 153 people from the Ukrainian population with the age from 0 to 101. Both models showed a high correlation with the chronological age in our study sample (R = 0.85 for the 2 CpG model and R = 0.92 for the 4 CpG model). We also estimated the accuracy metrics of the age prediction in our study sample. For the age group from 18 to 80 MAD was 5.1 years for the 2 CpG model and 4.1 years for the 4 CpG model. In this regard, we can conclude, that the models evaluated in the study have good age predictive accuracy, and can be used for the epigenetic age evaluation due to the relative simplicity and time-effectiveness.