A Multi-Catalytic Sensing for Hydrogen Peroxide, Glucose, and Organophosphorus Pesticides Based on Carbon Dots

• Published in: Frontiers in chemistry Vol. 9; p. 713104
• Main Authors: Li, Ping, Sun, Xiang-Ying, Shen, Jiang-Shan
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 12.07.2021
• Subjects: carbon dots (CDs); catalytic sensing; Chemistry; H2O2 and glucose sensing; organophosphorus (OPs) pesticide sensing; peroxidase-like catalytic activity; carbon dots (CDs); catalytic sensing; organophosphorus (OPs) pesticide sensing; H2O2 and glucose sensing; peroxidase-like catalytic activity
• ISSN: 2296-2646; 2296-2646
• DOI: 10.3389/fchem.2021.713104

In this work, a facile one-pot hydrothermal route was employed to synthesize a series of fluorescent carbon dots (CDs) by using 20 natural amino acids, respectively, as the starting materials. It was found that the CDs synthesized using phenylalanine could possess the intrinsic peroxidase-like activity that could effectively catalyze a traditional peroxidase substrate like 3, 3', 5, 5'- tetramethylbenzidine (TMB) in the presence of H O to produce a blue solution; thereby, a catalytic sensing system for H O has been developed. On the basis of this catalytic reaction, together with the fact that glucose oxidase (GOx) can catalyze the hydrolysis of glucose to generate H O , a sensitive catalytic sensing system for glucose could be further established. Furthermore, based on this catalytic reaction, taken together with the two enzymatic catalytic systems of acetylcholinesterase (AChE) and choline oxidase (CHO), a highly sensitive multi-catalytic sensing system could be successfully developed for organophosphorus (OPs) pesticides such as dimethoate, DDVP, and parathion-methyl. Limit of detections (LODs) of H O and glucose were estimated to be 6.5 and 0.84 μM, respectively. The limit of detection of the sub-nM level could be obtained for tested dimethoate, DDVP, and parathion-methyl OPs pesticides. The established sensing systems can exhibit good practical application performance in serum and several fruit samples.