Cross-species validation of human specific STR system, SureID ® 21G and SureID ® 23comp (Health Gene Technologies) in Chimpanzee (Pan Troglodytes)

• Published in: BMC research notes Vol. 12; no. 1; p. 750
• Main Authors: Singh, Abhishek, Thakur, Mukesh, Sahajpal, Vivek, Singh, Sujeet K, Chandra, Kailash, Sharma, Arun, Devi, Nisha, Bernot, Ausma
• Format: Journal Article
• Language: English
• Published: England BioMed Central 19.11.2019; BMC
• Subjects: Animals; Chimpanzees; Cross reactivity; DNA Fingerprinting - methods; Female; Gene Frequency; Genetics, Population; Genotyping Techniques; Haplotypes; Human identification; Humans; Inheritance Patterns; Male; Microsatellite Repeats; Pan troglodytes - genetics; Reagent Kits, Diagnostic - standards; Research Note; SureID® 21G; SureID® 23comp; SureID® 23comp; Chimpanzees; Human identification; SureID® 21G; Cross reactivity
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-019-4801-3

The human specific commercially available STRs system are often not tested in non human primates for their cross applicability. The aim of this study is to test Cross-species validation of two commercially available human specific STR kits i.e. SureID 21G and SureID 23comp (Health Gene Technologies) for their positive application in Chimpanzee (Pan troglodytes). In SureID 21G, 19 loci amplified and while 20 loci amplified in SureID 23comp. All the amplified loci in both STR kits were found polymorphic and the locus Amelogenin showed differential banding patterns between male and female revealing their known gender. The present study validates the applicability of these human specific STR kits in Chimpanzee that can be used in forensics analysis, paternity testing and population genetic studies.