High Rates of Genome Rearrangements and Pathogenicity of Shigella spp
are pathogens originating within the lineage but frequently classified as a separate genus. genomes contain numerous insertion sequences (ISs) that lead to pseudogenisation of affected genes and an increase of non-homologous recombination. Here, we study 414 genomes of and strains to assess the cont...
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Published in | Frontiers in microbiology Vol. 12; p. 628622 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
12.04.2021
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Subjects | |
Online Access | Get full text |
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Summary: | are pathogens originating within the
lineage but frequently classified as a separate genus.
genomes contain numerous insertion sequences (ISs) that lead to pseudogenisation of affected genes and an increase of non-homologous recombination. Here, we study 414 genomes of
and
strains to assess the contribution of genomic rearrangements to
evolution. We found that
experienced exceptionally high rates of intragenomic rearrangements and had a decreased rate of homologous recombination compared to pathogenic and non-pathogenic
. The high rearrangement rate resulted in independent disruption of syntenic regions and parallel rearrangements in different
lineages. Specifically, we identified two types of chromosomally encoded E3 ubiquitin-protein ligases acquired independently by all
strains that also showed a high level of sequence conservation in the promoter and further in the 5'-intergenic region. In the only available enteroinvasive
(EIEC) strain, which is a pathogenic
with a phenotype intermediate between
and non-pathogenic
, we found a rate of genome rearrangements comparable to those in other
and no functional copies of the two
-specific E3 ubiquitin ligases. These data indicate that the accumulation of ISs influenced many aspects of genome evolution and played an important role in the evolution of intracellular pathogens. Our research demonstrates the power of comparative genomics-based on synteny block composition and an important role of non-coding regions in the evolution of genomic islands. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Chih-Horng Kuo, Academia Sinica, Taiwan Reviewed by: Anne Kupczok, Wageningen University and Research, Netherlands; Hikaru Suenaga, National Institute of Advanced Industrial Science and Technology (AIST), Japan This article was submitted to Evolutionary and Genomic Microbiology, a section of the journal Frontiers in Microbiology |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2021.628622 |