Cytotoxic T-Cell Trafficking Chemokine Profiles Correlate With Defined Mucosal Microbial Communities in Colorectal Cancer

• Published in: Frontiers in immunology Vol. 12; p. 715559
• Main Authors: Zhang, Jiali, Tao, Ji, Gao, Ruo-Nan, Wei, Zhi-Yuan, He, Yu-Shan, Ren, Chun-Yan, Li, Qi-Chun, Liu, Yan-Shan, Wang, Ke-Wei, Yang, Gong, Qian, Chengjia, Chen, Jian-Huan
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 01.09.2021
• Subjects: adjacent normal tissues; Adult; Aged; Biomarkers; CD8-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - metabolism; chemokines; Chemokines - metabolism; Chemotaxis, Leukocyte - immunology; colorectal cancer; Colorectal Neoplasms - etiology; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; Computational Biology - methods; Disease Progression; Disease Susceptibility; Female; Fluorescent Antibody Technique; Gastrointestinal Microbiome - immunology; Humans; Immunohistochemistry; Immunology; Male; Metagenome; Metagenomics; Middle Aged; mucosa-associated bacteria; Neoplasm Grading; Neoplasm Staging; T cell trafficking; T-Lymphocytes, Cytotoxic - immunology; T-Lymphocytes, Cytotoxic - metabolism; tumor; colorectal cancer; mucosa-associated bacteria; T cell trafficking; adjacent normal tissues; chemokines; tumor
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2021.715559

The involvement of gut microbiota in T-cell trafficking into tumor tissue of colorectal cancer (CRC) remains to be further elucidated. The current study aimed to evaluate the expression of major cytotoxic T-cell trafficking chemokines (CTTCs) and chemokine-associated microbiota profiles in both tumor and adjacent normal tissues during CRC progression. We analyzed the expression of chemokine C-X-C motif ligands 9, 10, and 11 ( CXCL9 , CXCL10 , and CXCL11 ), and C-C motif ligand 5 ( CCL5 ), characterized gut mucosa-associated microbiota (MAM), and investigated their correlations in CRC patients. Our results showed that the expression of CXCL9, CXCL10 , and CXCL11 was significantly higher in tumor than in adjacent normal tissues in 136 CRC patients. Notably, the high expression of CXCL9 in tumor tissues was associated with enhanced CD8 + T-cell infiltration and improved survival. Moreover, the MAM in tumor tissues showed reduction of microbial diversity and increase of oral bacteria. Microbial network analysis identified differences in microbial composition and structure between tumor and adjacent normal tissues. In addition, stronger associations between oral bacteria and other gut microbes were observed. Furthermore, the correlation analysis between the defined MAM and individual CTTCs showed that the CTTCs’ correlated operational taxonomic units (OTUs) in tumor and adjacent normal tissues rarely overlap with each other. Notably, all the enriched OTUs were positively correlated with the CTTCs in either tumor or adjacent normal tissues. Our findings demonstrated stronger interactions between oral bacteria and gut microbes, and a shifted correlation pattern between MAM and major CTTCs in tumor tissues, underlining possible mechanisms of gut microbiota–host interaction in CRC.