Encapsulation of proteins in poly( l-lactide-co-caprolactone) fibers by emulsion electrospinning

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 75; no. 2; pp. 418 - 424
• Main Authors: Li, Xiaoqiang, Su, Yan, Liu, Shuiping, Tan, Lianjiang, Mo, Xiumei, Ramakrishna, Seeram
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2010
• Subjects: Animals; Cattle; Controlled release; Emulsion electrospinning; Emulsions; Humans; Microscopy, Electron, Scanning; Models, Chemical; Nerve Growth Factor - pharmacology; PC12 Cells; PLACL; Polyesters - chemistry; Rats; Serum Albumin, Bovine - chemistry; Solubility - drug effects; Tissue engineering; Tissue Engineering - methods; Water - chemistry; Emulsion electrospinning; PLACL; Tissue engineering; Controlled release
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2009.09.014

This study was aimed at investigating emulsion electrospinning to prepare biodegradable fibrous mats with encapsulation of human-nerve growth factor (NGF). One of the best methods for fabricating a bio-functional tissue engineering scaffold is to load bioactive agent into the scaffold. In this work, the feasibility of incorporating NGF into poly( l-lactide-co-caprolactone) fibers by emulsion electrospinning has been studied. The release behavior of encapsulated bovine serum albumin (BSA) was investigated. The bioactivity of NGF released from fibrous mats was verified by testing the neurite outgrowth of rat pheochromocytoma cells (PC12). Furthermore, the process of fiber forming during emulsion electrospinning was discussed. The results demonstrate that emulsion electrospun fibers can successfully encapsulate proteins and release them in a sustained manner. The bioactivity of NGF released from emulsion electrospun fibers was confirmed by PC12 bioassays.