7SK Acts as an Anti-tumor Factor in Tongue Squamous Cell Carcinoma

Increasing evidence has shown the mechanistic insights about non-coding RNA 7SK in controlling the transcription. However, the biological function and mechanism of 7SK in cancer are largely unclear. Here, we show that 7SK is down-regulated in human tongue squamous carcinoma (TSCC) and acts as a TSCC...

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Published inFrontiers in genetics Vol. 12; p. 642969
Main Authors Zhang, Bowen, Min, Sainan, Guo, Qi, Huang, Yan, Guo, Yuzhu, Liang, Xiaolin, Wu, Li-Ling, Yu, Guang-Yan, Wang, Xiangting
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 01.04.2021
Subjects
7SK
FOXJ3
Genetics
THRA
tongue squamous cell carcinoma
tumor suppressor
tumor suppressor
THRA
FOXJ3
tongue squamous cell carcinoma
7SK
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Summary:Increasing evidence has shown the mechanistic insights about non-coding RNA 7SK in controlling the transcription. However, the biological function and mechanism of 7SK in cancer are largely unclear. Here, we show that 7SK is down-regulated in human tongue squamous carcinoma (TSCC) and acts as a TSCC suppressor through multiple cell-based assays including a migration assay and a xenograft mouse model. The expression level of 7SK was negatively correlated with the size of tumors in the 73 in-house collected TSCC patients. Through combined analysis of 7SK knockdown of RNA-Seq and available published 7SK ChIRP-seq data, we identified 27 of 7SK-regulated genes that were involved in tumor regulation and whose upstream regulatory regions were bound by 7SK. Motif analysis showed that the regulatory sequences of these genes were enriched for transcription factors and , suggesting a potential involvement of and in 7SK-regulated genes. Interestingly, the augmented level of in TSCC patients and previous reports on in other cancers have suggested that these two factors may promote TSCC progression. In support of this idea, we found that 21 out of 27 aforementioned 7SK-associated genes were regulated by and , and 12 of them were oppositely regulated by 7SK and . We also found that and dramatically promoted migration in SCC15 cells. Collectively, we identified 7SK as an antitumor factor and suggested a potential involvement of and in 7SK-mediated TSCC progression.
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Edited by: Liang Chen, Wuhan University, China
This article was submitted to RNA, a section of the journal Frontiers in Genetics
Reviewed by: Xiong Ji, Peking University, China; Francesco Acquati, University of Insubria, Italy
ISSN:1664-8021
1664-8021
DOI:10.3389/fgene.2021.642969