Baicalin Alleviates LPS-Induced Oxidative Stress via NF-κB and Nrf2-HO1 Signaling Pathways in IPEC-J2 Cells

• Published in: Frontiers in veterinary science Vol. 8; p. 808233
• Main Authors: Bao, Minglong, Liang, Mei, Sun, Xinyi, Mohyuddin, Sahar Ghulam, Chen, Shengwei, Wen, Jiaying, Yong, Yanhong, Ma, Xingbin, Yu, Zhichao, Ju, Xianghong, Liu, Xiaoxi
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 25.01.2022
• Subjects: baicalin; inflammatory effect; lipopolysaccharide; NF-κB; Nrf2-HO-1; oxidative stress; Veterinary Science; NF-κB; Nrf2-HO-1; baicalin; lipopolysaccharide; oxidative stress; inflammatory effect
• ISSN: 2297-1769; 2297-1769
• DOI: 10.3389/fvets.2021.808233

Baicalin is a natural plant extract with anti-inflammatory and anti-oxidant activities. However, the molecular mechanism of baicalin on oxidative stress in IPEC-J2 cells exposed to LPS remains to be unclear. In this study, LPS stimulation significantly increased Toll-like receptor 4, tumor necrosis factor-α, and interleukins (IL-6 and IL-1β) expression in IPEC-J2 cells, and it activated the nuclear factor (NF-κB) expression. While, baicalin exerted anti-inflammatory effects by inhibiting NF-κB signaling pathway. LPS stimulation significantly increased the levels of the oxidative stress marker MDA, inhibited the anti-oxidant enzymes catalase and superoxide dismutase, which were all reversed by baicalin pre-treatment. It was found that baicalin treatment activated the nuclear import of nuclear factor-erythroid 2 related factor 2 (Nrf2) protein, and significantly increased the mRNA and protein expression of its downstream anti-oxidant factors such as heme oxygenase-1 and quinone oxidoreductase-1, which suggested that baicalin exerted anti-oxidant effects by activating the Nrf2-HO1 signaling pathway. Thus, pretreatment with baicalin inhibited LPS - induced oxidative stress and protected the normal physiological function of IPEC-J2 cells via NF-κB and Nrf2-HO1 signaling pathways.