Identification and Expression Analysis of WRKY Gene Family in Response to Abiotic Stress in Dendrobium catenatum
has become a rare and endangered medicinal plant due to habitat loss in China. As one of the most important and largest transcription factors, WRKY plays a critical role in response to abiotic stresses in plants. However, little is known regarding the functions of the WRKY family in . In this study,...
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Published in | Frontiers in genetics Vol. 13; p. 800019 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
03.02.2022
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Subjects | |
Online Access | Get full text |
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Summary: | has become a rare and endangered medicinal plant due to habitat loss in China. As one of the most important and largest transcription factors, WRKY plays a critical role in response to abiotic stresses in plants. However, little is known regarding the functions of the WRKY family in
. In this study, a total of 62
genes were identified from the
genome. Phylogenetic analysis revealed that DcWRKY proteins could be divided into three groups, a division supported by the conserved motif compositions and intron/exon structures.
gene expression and specific responses under drought, heat, cold and salt stresses were analyzed through RNA-seq data and RT-qPCR assay. The results showed that these genes had tissue-specificity and displayed different expression patterns in response to abiotic stresses. The expression levels of
,
and
were up-regulated by drought stress. Meanwhile,
was highly induced by heat in roots, and
was significantly induced by cold stress in leaves. Furthermore,
in roots and
in leaves were extremely induced under salt treatment. Finally, we found that all the five genes may function in ABA- and SA-dependent manners. This study identified candidate
genes with possible roles in abiotic stress and these findings not only contribute to our understanding of WRKY family genes, but also provide valuable information for stress resistance development in
. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Xiaojiao Han, Chinese Academy of Forestry, China Edited by: Sunil Kumar Sahu, Beijing Genomics Institute (BGI), China Reviewed by: Muhammad Waseem, Hainan University, China Muthusamy Ramakrishnan, Nanjing Forestry University, China This article was submitted to Plant Genomics, a section of the journal Frontiers in Genetics |
ISSN: | 1664-8021 1664-8021 |
DOI: | 10.3389/fgene.2022.800019 |