Taxonomic and Enzymatic Characterization of Flocculibacter collagenilyticus gen. nov., sp. nov., a Novel Gammaproteobacterium With High Collagenase Production
Collagens from marine animals are an important component of marine organic nitrogen. Collagenase-producing bacteria and their collagenases play important roles in collagen degradation and organic nitrogen recycling in the ocean. However, only a few collagenase-producing marine bacteria have been so...
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Published in | Frontiers in microbiology Vol. 12; p. 621161 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
09.03.2021
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Subjects | |
Online Access | Get full text |
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Summary: | Collagens from marine animals are an important component of marine organic nitrogen. Collagenase-producing bacteria and their collagenases play important roles in collagen degradation and organic nitrogen recycling in the ocean. However, only a few collagenase-producing marine bacteria have been so far discovered. Here, we reported the isolation and characterization of a collagenase-secreting bacterium, designated strain SM1988
, isolated from a green alga
sample. Strain SM1988
is a Gram-negative, aerobic, oxidase-, and catalase-positive, unipolar flagellated, and rod-shaped bacterium capable of hydrolyzing casein, gelatin and collagens. Phylogenetic analysis revealed that strain SM1988
formed a distinct phylogenetic lineage along with known genera within the family
, with 16S rRNA gene sequence similarity being less than 93.3% to all known species in the family. Based on the phylogenetic, genomic, chemotaxonomic and phenotypic data, strain SM1988
was considered to represent a novel species in a novel genus in the family
, for which the name
gen. nov., sp. nov. is proposed, with the type strain being SM1988
(= MCCC 1K04279
= KCTC 72761
). Strain SM1988
showed a high production of extracellular collagenases, which had high activity against both bovine collagen and codfish collagen. Biochemical tests combined with genome and secretome analyses indicated that the collagenases secreted by strain SM1988
are serine proteases from the MEROPS S8 family. These data suggest that strain SM1988
acts as an important player in marine collagen degradation and recycling and may have a promising potential in collagen resource utilization. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Martin G. Klotz, Washington State University, United States This article was submitted to Aquatic Microbiology, a section of the journal Frontiers in Microbiology Reviewed by: Jung-Sook Lee, Korea Research Institute of Bioscience and Biotechnology (KRIBB), South Korea; Ulrich Eckhard, Instituto de Biología Molecular de Barcelona (IBMB), Spain; Andrew Charles Doxey, University of Waterloo, Canada |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2021.621161 |