Overexpression of Global Regulator Talae1 Leads to the Discovery of New Antifungal Polyketides From Endophytic Fungus Trichoderma afroharzianum

Transcription regulation caused by global regulators exerts important effects on fungal secondary metabolism. By overexpression of the global regulator Talae1 in a -associated fungus , two structurally new polyketides ( and ) that were newly produced in the transformant were isolated and identified....

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Published inFrontiers in microbiology Vol. 11; p. 622785
Main Authors Ding, Zhuang, Wang, Xiao, Kong, Fan-Dong, Huang, Hui-Ming, Zhao, Yan-Na, Liu, Min, Wang, Zheng-Ping, Han, Jun
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 23.12.2020
Subjects
antifungal activity
endophytic fungi
global regulator
LaeA
Microbiology
polyketides
Trichoderma afroharzianum
Trichoderma afroharzianum
global regulator
polyketides
LaeA
antifungal activity
endophytic fungi
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Summary:Transcription regulation caused by global regulators exerts important effects on fungal secondary metabolism. By overexpression of the global regulator Talae1 in a -associated fungus , two structurally new polyketides ( and ) that were newly produced in the transformant were isolated and identified. Their structures, including the absolute configurations, were elucidated through a combination of high resolution mass spectrometer (HRMS), NMR, and electronic circular dichroism (ECD) calculations. The growth inhibitory activities of compounds and were evaluated against four bacteria and six plant-pathogenic fungi. Compound showed the highest antifungal activity against and f. sp. with MIC of 8 μg/ml. To the best of our knowledge, this is the first study to report on the application of the global regulator in to activate the biosynthesis of bioactive secondary metabolites.
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This article was submitted to Microbiotechnology, a section of the journal Frontiers in Microbiology
Reviewed by: Weaam Ebrahim, Mansoura University, Egypt; Liwei Wang, Hangzhou Normal University, China; Chunyuan Li, South China Agricultural University, China
Edited by: Paola Angelini, University of Perugia, Italy
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2020.622785