Clinical signs and bacterial communities of deciduous necrotic root canals detected by PCR-DGGE analysis: Research association

Abstract Objective This study sought to investigate the possible association between clinical and radiographic data of the patients with the bacterial community profiles involved in cases of necrosis in primary root canals. Methods Microbial community profiles for 25 samples from necrotic deciduous...

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Published inArchives of oral biology Vol. 59; no. 8; pp. 848 - 854
Main Authors de Paula, Viviane Andrade Cancio, de Carvalho Ferreira, Dennis, Cavalcante, Fernanda Sampaio, do Carmo, Flávia Lima, Rosado, Alexandre Soares, Primo, Laura Guimarães, dos Santos, Kátia Regina Netto
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.08.2014
Subjects
Advanced Basic Science
Bacteria
Bacterial Typing Techniques
Child
Child, Preschool
Deciduous root canals
Denaturing Gradient Gel Electrophoresis
Dental Pulp Cavity - microbiology
Dentistry
Endodontic infection
Female
Humans
Infant
Male
Necrosis - microbiology
Periapical Diseases - microbiology
Polymerase Chain Reaction
Primary teeth
Root Canal Therapy
Tooth, Deciduous - microbiology
Denaturing gradient gel electrophoresis
Primary teeth
Deciduous root canals
Endodontic infection
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Summary:Abstract Objective This study sought to investigate the possible association between clinical and radiographic data of the patients with the bacterial community profiles involved in cases of necrosis in primary root canals. Methods Microbial community profiles for 25 samples from necrotic deciduous root canals were analyzed using the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting approach. These results were related to the clinical and radiographic data of these patients. Results The analysis showed a large diversity of microbial communities in necrotic deciduous root canals. The statistical results pointed out that posterior and anterior teeth were associated with <20 bands and >20 bands in PCR-DGGE method, respectively. A relationship was verified between ages >4 years old and posterior teeth and, ages ≤4 years old and anterior teeth. Conclusions The data showed a polymicrobial community and pointed out the association of age with necrosis in anterior and posterior teeth.
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ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2014.05.012