Induction of Functional Specific Antibodies, IgG-Secreting Plasmablasts and Memory B Cells Following BCG Vaccination

• Published in: Frontiers in immunology Vol. 12; p. 798207
• Main Authors: Bitencourt, Julia, Peralta-Álvarez, Marco Polo, Wilkie, Morven, Jacobs, Ashley, Wright, Daniel, Salman Almujri, Salem, Li, Shuailin, Harris, Stephanie A, Smith, Steven G, Elias, Sean C, White, Andrew D, Satti, Iman, Sharpe, Sally S, O'Shea, Matthew K, McShane, Helen, Tanner, Rachel
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 05.01.2022
• Subjects: Adult; Animals; antibodies; Antibodies, Bacterial - blood; Antibodies, Bacterial - immunology; B cells; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; BCG; BCG Vaccine - administration & dosage; BCG Vaccine - immunology; Cells, Cultured; Enzyme-Linked Immunosorbent Assay - methods; Female; Humans; humoral immunity; Immunity, Cellular - drug effects; Immunity, Cellular - immunology; Immunity, Humoral - drug effects; Immunity, Humoral - immunology; Immunoglobulin A - blood; Immunoglobulin A - immunology; Immunoglobulin G - blood; Immunoglobulin G - immunology; Immunoglobulin G - metabolism; Immunoglobulin M - blood; Immunoglobulin M - immunology; Immunology; Macaca fascicularis - immunology; Macaca mulatta - immunology; Male; Memory B Cells - immunology; Memory B Cells - metabolism; Mycobacterium tuberculosis - immunology; Mycobacterium tuberculosis - physiology; Plasma Cells - immunology; Plasma Cells - metabolism; tuberculosis; Tuberculosis - immunology; Tuberculosis - microbiology; Tuberculosis - prevention & control; Vaccination - methods; humoral immunity; vaccine; BCG; tuberculosis; antibodies; B cells; TB
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2021.798207

Tuberculosis (TB) is a major global health problem and the only currently-licensed vaccine, BCG, is inadequate. Many TB vaccine candidates are designed to be given as a boost to BCG; an understanding of the BCG-induced immune response is therefore critical, and the opportunity to relate this to circumstances where BCG does confer protection may direct the design of more efficacious vaccines. While the T cell response to BCG vaccination has been well-characterized, there is a paucity of literature on the humoral response. We demonstrate BCG vaccine-mediated induction of specific antibodies in different human populations and macaque species which represent important preclinical models for TB vaccine development. We observe a strong correlation between antibody titers in serum versus plasma with modestly higher titers in serum. We also report for the first time the rapid and transient induction of antibody-secreting plasmablasts following BCG vaccination, together with a robust and durable memory B cell response in humans. Finally, we demonstrate a functional role for BCG vaccine-induced specific antibodies in opsonizing mycobacteria and enhancing macrophage phagocytosis , which may contribute to the BCG vaccine-mediated control of mycobacterial growth observed. Taken together, our findings indicate that the humoral immune response in the context of BCG vaccination merits further attention to determine whether TB vaccine candidates could benefit from the induction of humoral as well as cellular immunity.