A carlactonoic acid methyltransferase that contributes to the inhibition of shoot branching in Arabidopsis
SignificanceStrigolactones (SLs) are a group of apocarotenoid hormones, which regulates shoot branching and other diverse developmental processes in plants. The major bioactive form(s) of SLs as endogenous hormones has not yet been clarified. Here, we identify an methyltransferase, CLAMT, responsibl...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 119; no. 14; p. e2111565119 |
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Main Authors | , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
05.04.2022
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Subjects | |
Online Access | Get full text |
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Summary: | SignificanceStrigolactones (SLs) are a group of apocarotenoid hormones, which regulates shoot branching and other diverse developmental processes in plants. The major bioactive form(s) of SLs as endogenous hormones has not yet been clarified. Here, we identify an
methyltransferase, CLAMT, responsible for the conversion of an inactive precursor to a biologically active SL that can interact with the SL receptor in vitro. Reverse genetic analysis showed that this enzyme plays an essential role in inhibiting shoot branching. This mutant also contributed to specifying the SL-related metabolites that could move from root to shoot in grafting experiments. Our work has identified a key enzyme necessary for the production of the bioactive form(s) of SLs. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions: K.M., Y.S., H.B., and S.Y. designed research; K.M., Y.S., Y.O., S.S., K.T., Y.W., L.D., K. Asami, R.N., T.K., N.K., and K. Akiyama performed research; K.M., Y.S., Y.O., S.S., K.T., Y.W., L.D., K. Asami, R.N., T.K., N.K., K. Akiyama, H.B, and S.Y. analyzed data; and K.M., Y.S., H.B., and S.Y. wrote the paper. 1K.M., Y.S., and Y.O. contributed equally to this work. 2Present address: Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo, Hokkaido, 060-0808, Japan. Edited by Bonnie Bartel, Rice University, Houston, TX; received June 28, 2021; accepted February 18, 2022 |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.2111565119 |