YgiM may act as a trigger in the sepsis caused by Klebsiella pneumoniae through the membrane-associated ceRNA network
Sepsis is one of the diseases that can cause serious mortality. In , an inner membrane protein YgiM encoded by gene can target the eukaryotic peroxisome. Peroxisome is a membrane-enclosed organelle associated with the ROS metabolism and was reported to play the key role in immune responses and infla...
Saved in:
Published in | Frontiers in genetics Vol. 13; p. 973145 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
23.09.2022
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Sepsis is one of the diseases that can cause serious mortality. In
, an inner membrane protein YgiM encoded by gene
can target the eukaryotic peroxisome. Peroxisome is a membrane-enclosed organelle associated with the ROS metabolism and was reported to play the key role in immune responses and inflammation during the development of sepsis.
(
) is one of the important pathogens causing sepsis. However, the function of gene
which is highly homologous to
of
has not been demonstrated in
. In this study, we prepared Δ
of
ATCC43816, and found that the deletion of
did not affect bacterial growth and mouse mortality in the mouse infection model. Interestingly, Δ
not only resulted in reduced bacterial resistance to macrophages, but also attenuated pathological manifestations in mouse organs. Furthermore, based on the data of Gene Expression Omnibus, the expression profiles of micro RNAs (miRNAs) and messenger RNAs (mRNAs) in the serum of 44 sepsis patients caused by
infection were analyzed, and 11 differently expressed miRNAs and 8 DEmRNAs associated with the membrane function were found. Finally, the membrane-associated competing endogenous RNAs (ceRNAs) network was constructed. In this ceRNAs network, DEmiRNAs (hsa-miR-7108-5p, hsa-miR-6780a-5p, hsa-miR-6756-5p, hsa-miR-4433b-3p, hsa-miR-3652, hsa-miR-342-3p, hsa-miR-32-5p) and their potential downstream target DEmRNAs (VNN1, CEACAM8, PGLYRP1) were verified in the cell model infected by wild type and Δ
of
respectively. Taken together, YgiM may trigger the sepsis caused by
via membrane-associated ceRNAs. This study provided new insights into the role of YgiM in the process of
induced sepsis. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Qi Dai, Zhejiang Sci-Tech University, China Edited by: Jade Ll Teng, The University of Hong Kong, Hong Kong SAR, China These authors have contributed equally to this work Hwei Huih Lee, The University of Hong Kong, Hong Kong SAR, China This article was submitted to RNA, a section of the journal Frontiers in Genetics |
ISSN: | 1664-8021 1664-8021 |
DOI: | 10.3389/fgene.2022.973145 |