YgiM may act as a trigger in the sepsis caused by Klebsiella pneumoniae through the membrane-associated ceRNA network

• Published in: Frontiers in genetics Vol. 13; p. 973145
• Main Authors: Han, Mingxiao, Chen, Zhihao, He, Ping, Li, Ziyuan, Chen, Qi, Tong, Zelei, Wang, Min, Du, Hong, Zhang, Haifang
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 23.09.2022
• Subjects: Genetics; Klebsiella pneumoniae; miRNA; mRNA; peroxisome; sepsis; ygiM; ceRNAs network; miRNA; peroxisome; mRNA; ygiM; Klebsiella pneumoniae; sepsis
• ISSN: 1664-8021; 1664-8021
• DOI: 10.3389/fgene.2022.973145

Sepsis is one of the diseases that can cause serious mortality. In , an inner membrane protein YgiM encoded by gene can target the eukaryotic peroxisome. Peroxisome is a membrane-enclosed organelle associated with the ROS metabolism and was reported to play the key role in immune responses and inflammation during the development of sepsis. ( ) is one of the important pathogens causing sepsis. However, the function of gene which is highly homologous to of has not been demonstrated in . In this study, we prepared Δ of ATCC43816, and found that the deletion of did not affect bacterial growth and mouse mortality in the mouse infection model. Interestingly, Δ not only resulted in reduced bacterial resistance to macrophages, but also attenuated pathological manifestations in mouse organs. Furthermore, based on the data of Gene Expression Omnibus, the expression profiles of micro RNAs (miRNAs) and messenger RNAs (mRNAs) in the serum of 44 sepsis patients caused by infection were analyzed, and 11 differently expressed miRNAs and 8 DEmRNAs associated with the membrane function were found. Finally, the membrane-associated competing endogenous RNAs (ceRNAs) network was constructed. In this ceRNAs network, DEmiRNAs (hsa-miR-7108-5p, hsa-miR-6780a-5p, hsa-miR-6756-5p, hsa-miR-4433b-3p, hsa-miR-3652, hsa-miR-342-3p, hsa-miR-32-5p) and their potential downstream target DEmRNAs (VNN1, CEACAM8, PGLYRP1) were verified in the cell model infected by wild type and Δ of respectively. Taken together, YgiM may trigger the sepsis caused by via membrane-associated ceRNAs. This study provided new insights into the role of YgiM in the process of induced sepsis.