Effects of juvenile hormone (JH) analog insecticides on larval development and JH esterase activity in two spodopterans

• Published in: Pesticide biochemistry and physiology Vol. 128; pp. 30 - 36
• Main Authors: El-Sheikh, El-Sayed A., Kamita, Shizuo G., Hammock, Bruce D.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2016
• Subjects: Animals; Carboxylic Ester Hydrolases - metabolism; Inhibition; Insecticides - pharmacology; JH esterase; Juvenile hormone (JH) analog insecticide; Juvenile Hormones - pharmacology; Larva - drug effects; Larva - growth & development; S. frugiperda; Spodoptera - growth & development; Spodoptera frugiperda; Spodoptera littoralis; JH esterase; Inhibition; Juvenile hormone (JH) analog insecticide; Spodoptera littoralis; S. frugiperda
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2015.10.008

Juvenile hormone analog (JHA) insecticides are biological and structural mimics of JH, a key insect developmental hormone. Toxic and anti-developmental effects of the JHA insecticides methoprene, fenoxycarb, and pyriproxyfen were investigated on the larval and pupal stages of Spodoptera littoralis and Spodoptera frugiperda. Bioassays showed that fenoxycarb has the highest toxicity and fastest speed of kill in 2nd instar S. littoralis. All three JHAs affected the development of 6th instar (i.e., final instar) and pupal S. frugiperda. JH esterase (JHE) is a critical enzyme that helps to regulate JH levels during insect development. JHE activity in the last instar S. littoralis and S. frugiperda was 11 and 23nmolmin−1ml−1 hemolymph, respectively. Methoprene and pyriproxyfen showed poor inhibition of JHE activity from these insects, whereas fenoxycarb showed stronger inhibition. The inhibitory activity of fenoxycarb, however, was more than 1000-fold lower than that of OTFP, a highly potent inhibitor of JHEs. Surprisingly, topical application of methoprene, fenoxycarb or pyriproxyfen on 6th instars of S. littoralis and S. frugiperda prevented the dramatic reduction in JHE activity that was found in control insects. Our findings suggest that JHAs may function as JH agonists that play a disruptive role or a hormonal replacement role in S. littoralis and S. frugiperda. [Display omitted] •Topical application of methoprene, fenoxycarb or pyriproxyfen significantly extends the larval stage of S. frugiperda.•In vitro JHE activity is inhibited by fenoxycarb in competition experiments with JH III.•Hemolymph JHE activity is maintained by methoprene, fenoxycarb or pyriproxyfen treatment of 6th instar S. frugiperda.