The complete chloroplast genome of Hibiscus syriacus using long-read sequencing: Comparative analysis to examine the evolution of the tribe Hibisceae

, a member of the tribe Hibisceae, is considered an important ornamental and medicinal plant in east Asian countries. Here, we sequenced and assembled the complete chloroplast genome of var. using the PacBio long-read sequencing platform. A quadripartite structure with 161,026 base pairs was obtaine...

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Published inFrontiers in plant science Vol. 14; p. 1111968
Main Authors Koo, Hyunjin, Shin, Ah-Young, Hong, Seongmin, Kim, Yong-Min
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 02.02.2023
Subjects
comparative analysis
complete chloroplast genome assembly
Hibisceae
Hibiscus syriacus
long-read sequencing platform
Plant Science
Hibiscus syriacus
comparative analysis
Hibisceae
complete chloroplast genome assembly
long-read sequencing platform
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Summary:, a member of the tribe Hibisceae, is considered an important ornamental and medicinal plant in east Asian countries. Here, we sequenced and assembled the complete chloroplast genome of var. using the PacBio long-read sequencing platform. A quadripartite structure with 161,026 base pairs was obtained, consisting of a pair of inverted repeats (IRA and IRB) with 25,745 base pairs, separated by a large single-copy region of 89,705 base pairs and a short single-copy region of 19,831 base pairs. This chloroplast genome had 79 protein-coding genes, 30 transfer RNA genes, 4 ribosomal RNA genes, and 109 simple sequence repeat regions. Among them, and , containing traces of RNA-editing events associated with adaptive evolution, were identified by analysis of putative RNA-editing sites. Codon usage analysis revealed a preference for A/U-terminated codons. Furthermore, the codon usage pattern had a clustering tendency similar to that of the phylogenetic analysis of the tribe Hibisceae. This study provides clues for understanding the relationships and refining the taxonomy of the tribe Hibisceae.
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This article was submitted to Plant Systematics and Evolution, a section of the journal Frontiers in Plant Science
These authors have contributed equally to this work
Reviewed by: Diaga Diouf, Cheikh Anta Diop University, Senegal; Muhammad Aamir Manzoor, Anhui Agricultural University, China
Edited by: Magdy S. Alabady, University of Georgia, United States
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2023.1111968