Forty percent and eighty percent methionine restriction decrease mitochondrial ROS generation and oxidative stress in rat liver

• Published in: Biogerontology (Dordrecht) Vol. 9; no. 3; pp. 183 - 196
• Main Authors: Caro, Pilar, Gómez, José, López-Torres, Mónica, Sánchez, Inés, Naudí, Alba, Jove, Mariona, Pamplona, Reinald, Barja, Gustavo
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2008; Springer; Springer Nature B.V
• Subjects: Aging; Animals; Apoptosis; Biological and medical sciences; Biomedical and Life Sciences; Carbohydrates; Cell Biology; Development. Metamorphosis. Moult. Ageing; Developmental Biology; Diet; DNA, Mitochondrial - metabolism; Free radicals; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; Geriatrics/Gerontology; Life Sciences; Lipids; Liver; Male; Methionine - administration & dosage; Mitochondria; Mitochondria, Liver - metabolism; Mitochondrial DNA; Oxidation; Oxidative Stress; Proteins; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism; Research Article; Rodents; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Oxygen radicals; Mitochondria; Respiratory complexes; Aging; Longevity; Protein damage; Fatty acids; Caloric restriction; Oxidative stress; Fatty acids .Respiratory complexes; Thiol; Oxygen; Senescence; Rat; Digestive system; Liver; Ageing; Mitochondria .Caloric restriction; Rodentia; Methionine; Lipids; Protein; Sulfur containing aminoacid; Vertebrata; Generation; Mammalia
• ISSN: 1389-5729; 1573-6768
• DOI: 10.1007/s10522-008-9130-1

Dietary restriction (DR) lowers mitochondrial reactive oxygen species (ROS) generation and oxidative damage and increases maximum longevity in rodents. Protein restriction (PR) or methionine restriction (MetR), but not lipid or carbohydrate restriction, also cause those kinds of changes. However, previous experiments of MetR were performed only at 80% MetR, and substituting dietary methionine with glutamate in the diet. In order to clarify if MetR can be responsible for the lowered ROS production and oxidative stress induced by standard (40%) DR, Wistar rats were subjected to 40% or 80% MetR without changing other dietary components. It was found that both 40% and 80% MetR decrease mitochondrial ROS generation and percent free radical leak in rat liver mitochondria, similarly to what has been previously observed in 40% PR and 40% DR. The concentration of complexes I and III, apoptosis inducing factor, oxidative damage to mitochondrial DNA, five different markers of protein oxidation, glycoxidation or lipoxidation and fatty acid unsaturation were also lowered. The results show that 40% isocaloric MetR is enough to decrease ROS production and oxidative stress in rat liver. This suggests that the lowered intake of methionine is responsible for the decrease in oxidative stress observed in DR.