Whole-Genome Sequencing of Bradyrhizobium diazoefficiens 113-2 and Comparative Genomic Analysis Provide Molecular Insights Into Species Specificity and Host Specificity
In the present study, we sequenced the complete genome of 113-2. The genomic characteristics of six selected rhizobial strains (two fast-growing rhizobia, two medium-slow-growing rhizobia and two slow-growing rhizobia) with four different legume hosts were analyzed by comparative genomic analysis. G...
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Published in | Frontiers in microbiology Vol. 11; p. 576800 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
16.11.2020
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Subjects | |
Online Access | Get full text |
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Summary: | In the present study, we sequenced the complete genome of
113-2. The genomic characteristics of six selected rhizobial strains (two fast-growing rhizobia, two medium-slow-growing rhizobia and two slow-growing rhizobia) with four different legume hosts were analyzed by comparative genomic analysis. Genomes of
113-2 and
USDA110 were found to share a large synteny blocks and a high ANI value, supporting 113-2 as a strain of
. 5,455 singletons and 11,656 clusters were identified among the six rhizobia genomes, and most of the pair-wise comparisons clusters were shared by the two genomes of strains in the same genus. Similar genus-specific gene numbers in the assigned COG functional terms were present in the two strains of the same genus, while the numbers were decreased with the increase of growth rate in most of the COG terms. KEGG pathway analysis of
113-2 suggested that the rhizobial genes in ABC transporters and Two-Component system were mainly species-specific. Besides, the candidate genes related to secretion system and surface polysaccharides biosynthesis in the genomes of the six strains were explored and compared. 39 nodulation gene families, 12
gene families and 10
gene families in the genomes of these six strains were identified, and gene classes in most of gene families and the types and total gene numbers of gene families were substantially different among these six genomes. We also performed synteny analyses for above-mentioned
,
, and
gene groupings, and selected
,
,
,
,
, and
gene families to perform phylogeny analyses. Our results provided valuable molecular insights into species specificity and host specificity. The genetic information responsible for host specificity will play important roles in expanding the host range of rhizobia among legumes, which might provide new clues for the understanding of the genetic determinants of non-legume-rhizobium symbiosis. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors have contributed equally to this work This article was submitted to Microbial Symbioses, a section of the journal Frontiers in Microbiology Edited by: Gustavo Santoyo, Universidad Michoacana de San Nicolás de Hidalgo, Mexico Reviewed by: Elena Erika Fedorova, Timiryazev Institute of Plant Physiology RAS, Russia; Luis Rey, Polytechnic University of Madrid, Spain |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2020.576800 |