Clonal Relationship and Resistance Profiles Among ESBL-Producing Escherichia coli

• Published in: Frontiers in cellular and infection microbiology Vol. 11; p. 560622
• Main Authors: Dolatyar Dehkharghani, Alireza, Haghighat, Setareh, Rahnamaye Farzami, Marjan, Rahbar, Mohammad, Douraghi, Masoumeh
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 23.06.2021
• Subjects: AmpC; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Bacterial Proteins - genetics; beta-Lactamases - genetics; Cellular and Infection Microbiology; E. coli; Escherichia coli - genetics; Escherichia coli Infections - drug therapy; gene; Humans; Microbial Sensitivity Tests; molecular typing; resistance; Urinary Tract Infections; AmpC; gene; E. coli; resistance; molecular typing
• ISSN: 2235-2988; 2235-2988
• DOI: 10.3389/fcimb.2021.560622

AmpC β-lactamases hydrolyze all β-lactams except cefepime and carbapenems. The study of AmpC-producing has high priority for the infection control committee. This research is aimed to investigate the resistant urinary AmpC-generating isolates and identify their genetic variety. Some 230 isolates from patients suffering urinary tract infection symptoms were studied in 2017-2018 to assess their susceptibility toward antimicrobial agents. AmpC gene was evaluated by PCR and molecular typing using the 10-loci MLVA method. MLVA images were examined by BioNumerics 6.6 software through the use of the UPGMA algorithms. Thirty-eight AmpC-generating isolates were detected. The most abundant determinant was and , , and had the next ranks, respectively. Six major clusters and a singleton were identified by MLVA. AmpC beta-lactamases in urinary isolates of in the hospital under study and high rate of additional resistance to gentamicin, cotrimoxazole and ciprofloxacin. The most frequent gene determinant of AmpC beta-lactamase was and vary depending on time and geographical location.