Immunohistochemical localization and pharmacokinetics of the anti-MRSA drug teicoplanin in rat kidney using a newly developed specific antibody

• Published in: Medical molecular morphology Vol. 54; no. 3; pp. 227 - 236
• Main Authors: Yamamoto, Yutaro, Yamamoto, Yuta, Saita, Tetsuya, Shin, Masashi
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.09.2021; Springer Nature B.V
• Subjects: Anatomy; Animals; Anti-Bacterial Agents; Antibodies; Bovine serum albumin; Cytoplasm; Distal tubules; Diuretics; Drug resistance; Epithelial cells; Immunohistochemistry; Immunohistochemistry - methods; Injection; Injections, Intravenous; Intravenous administration; Kidney - metabolism; Kidneys; Localization; Medicine; Medicine & Public Health; Molecular Medicine; Original Paper; Pathology; Pharmacokinetics; Polyclonal antibodies; Rats; Teicoplanin; Teicoplanin - administration & dosage; Teicoplanin - analysis; Teicoplanin - immunology; Teicoplanin - pharmacokinetics; Vancomycin; Immunohistochemistry; Adverse event; Pharmacokinetics; Teicoplanin; Kidney
• ISSN: 1860-1480; 1860-1499
• DOI: 10.1007/s00795-021-00286-6

We prepared a polyclonal antibody against a teicoplanin (TEIC)-bovine serum albumin conjugate that was specific to both conjugated and free forms of TEIC. We demonstrated that this antibody could be used to detect the time-dependent localization of TEIC in rat kidneys. Immunohistochemistry revealed immunoreactivity specifically in the microvilli and apical cytoplasm of epithelial cells in proximal tubule segments S1 and S2, 1 h after intravenous TEIC injection, with higher staining intensity in the S2 segments. The epithelial cells of S3 segments showed moderate immunostaining with a few cells exhibiting nuclear staining. Furthermore, we found that the distal tubules and collecting ducts contained both TEIC-positive and -negative cells. TEIC immunoreactivity decreased rapidly over time; only weak staining remained in the S3 segments, distal tubules, and collecting ducts 24 h after administration. No staining was detected 7 days after injection. These results were significantly different from those of our previous study obtained using vancomycin, which showed moderate staining in the proximal tubule segments S1 and S2, distal tubules, and the collecting ducts 8 days after administration. The lower TEIC accumulation in tissues may account for a lower risk of adverse events compared to that using vancomycin.