Understanding the Transcriptional Changes During Infection of Meloidogyne incognita Eggs by the Egg-Parasitic Fungus Purpureocillium lilacinum

• Published in: Frontiers in microbiology Vol. 12; p. 617710
• Main Authors: Xu, Wen-Feng, Yang, Jia-Lin, Meng, Xiang-Kun, Gu, Zhi-Guang, Zhang, Qi-Lin, Lin, Lian-Bing
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 07.04.2021
• Subjects: gene expression; Meloidogyne incognita eggs; Microbiology; Nematophagous fungus; Purpureocillium lilacinum; RNA-seq; transcriptomics; transcriptomics; Meloidogyne incognita eggs; Nematophagous fungus; RNA-seq; Purpureocillium lilacinum; gene expression
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2021.617710

The egg-pathogenic fungus parasitizes on nematode eggs, and thus, it is used as a good biocontrol agent against plant root-knot nematodes. However, little is known about the transcriptional response of while infecting nematode eggs. This study presents the whole transcriptome sequencing of and transcriptome-wide gene expression analysis of upon infecting the eggs of compared to non-infecting controls. A transcriptomic library of was used as reference gene set and six transcriptomic libraries of the non-infecting control and infecting eggs were constructed, respectively, comprising three biological replicates of each. A total of 1,011 differently expressed genes (DEGs) were identified in the infecting samples, including 553 up-regulated and 458 down-regulated genes compared to the non-infecting control samples. Furthermore, functional enrichment analysis exhibited that these DEGs were primarily involved in oxidative phosphorylation, oxidoreductase activity, and metabolic processes. Fifteen DEGs were randomly selected to verify the RNA sequencing results through quantitative real-time polymerase chain reaction (qPCR). The study focused on genes that were strongly expressed upon infecting eggs. These DEGs were primarily involved in detoxification, parasitic behavior, and nutritional utilization. This study contributes significantly to the understanding of the molecular mechanisms underlying the parasitic action of on nematode eggs and provides a valuable genetic resource for further research on parasitic behavior of . Notably, this study examined the transcriptomics of infecting eggs at only one time point. Since there were fungi at different stages of the infection process at that time point, the transcriptional profiles are not precisely examining one specific stage in this process.