Transcriptomic Profiling Analysis of Castration-Resistant Prostate Cancer Cell Lines Treated with Chronic Intermittent Hypoxia

Castration-resistant prostate cancer (CRPC) is still a major concern in men’s health, with 375,000 cancer deaths annually. Hypoxia, which is a marked characteristic of advanced solid tumors, has been suggested to induce prostate cancer towards CRPC, metastasis and treatment resistance. To evaluate t...

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Published inCancers Vol. 14; no. 16; p. 3959
Main Authors Lee, Chung Lyul, Lee, Minji, Lee, Ji Yong, Hong, Sin-hyoung, Yang, Seung Woo, Min, Ji-hyeon, Lee, Dong-eon, Baek, Joonyoung, Kim, Chanseul, Lim, Jae Sung, Song, Ki Hak, Shin, Ju Hyun, Kim, Gun-Hwa
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.08.2022
MDPI
Subjects
Androgens
Bone cancer
Care and treatment
Castration
Cell culture
Cell migration
Datasets
Gene expression
Genetic aspects
Health aspects
Hypoxia
Metastases
Metastasis
Patients
Prostate cancer
Proteins
Ribonucleic acid
RNA
Sequence analysis
Solid tumors
Transcriptomics
Tumor cell lines
Wound healing
South Korea
United States > US
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Summary:Castration-resistant prostate cancer (CRPC) is still a major concern in men’s health, with 375,000 cancer deaths annually. Hypoxia, which is a marked characteristic of advanced solid tumors, has been suggested to induce prostate cancer towards CRPC, metastasis and treatment resistance. To evaluate the effect of hypoxia on prostate cancer, two and five cycles of hypoxia and reoxygenation were administered using 22Rv1 cell lines and denominated as 22Rv1-CI and 22Rv1-PCI, respectively. Cancer cell migration was promoted in 22Rv1-CI compared to controls, and the expression of COL13A1 was significantly up-regulated in 22Rv1-CI according to differentially expressed gene analysis of RNA sequencing among groups. Cancer cell migration was impeded in a wound healing assay after transfecting si-COL13A1. Moreover, the expression of COL13A1 was also higher in the cell line originating from bone metastatic prostate cancer compared to other cell lines. Using the open database GEO, we also confirmed that the expression of COL13A1 was higher in bone metastatic prostate cancer tissue than in localized prostate cancer tissue in patients. Therefore, COL13A1 may be closely related to the bony metastasis of prostate cancer, and our findings may provide valuable information on the pathophysiology of the metastatic niche induced by hypoxia in patients with CRPC.
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These authors contributed equally to this work.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers14163959