Genetic Variants at PSMD3 Interact with Dietary Fat and Carbohydrate to Modulate Insulin Resistance

• Published in: The Journal of nutrition Vol. 143; no. 3; pp. 354 - 361
• Main Authors: Zheng, Ju-Sheng, Arnett, Donna K., Parnell, Laurence D., Lee, Yu-Chi, Ma, Yiyi, Smith, Caren E., Richardson, Kris, Li, Duo, Borecki, Ingrid B., Ordovas, Jose M., Tucker, Katherine L., Lai, Chao-Qiang
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01.03.2013; American Society for Nutrition
• Subjects: Adult; Aged; Alleles; Biological and medical sciences; Blood Glucose - genetics; Blood Glucose - metabolism; Boston; Dietary Carbohydrates - metabolism; Dietary Fats - metabolism; Fatty Acids, Monounsaturated - metabolism; Feeding. Feeding behavior; Female; Fundamental and applied biological sciences. Psychology; Hispanic or Latino; Homozygote; Humans; Insulin - genetics; Insulin - metabolism; Insulin Resistance - genetics; Male; Middle Aged; Nutritional Epidemiology; Polymorphism, Single Nucleotide; Proteasome Endopeptidase Complex - genetics; Proteasome Endopeptidase Complex - metabolism; Sex Factors; Signal Transduction; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Boston; HOMA-IR; GWAS; GOLDN; MAF; SNP; BPRHS; LD; IR; WBC; Endocrinopathy; Target tissue resistance; Nutrition; Genetic variant; Fat; Metabolic diseases; Insulin resistance; Carbohydrate
• ISSN: 0022-3166; 1541-6100; 1541-6100
• DOI: 10.3945/jn.112.168401

PSMD3 encodes subunit 3 of the 26S proteasome, which is involved in regulating insulin signal transduction, and dietary factors could potentially regulate the function of this gene. We aimed to investigate the associations of PSMD3 variants with glucose-related traits and the interactions of those variants with dietary fat and carbohydrate for glucose-related traits in the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN) study and to replicate the findings in the Boston Puerto Rican Health Study (BPRHS). Ten single nucleotide polymorphisms (SNPs) were selected, covering 90% the genetic variations in or near PSMD3. Minor allele (C) carriers of rs4065321 had higher homeostasis model assessment of insulin resistance (HOMA-IR) than noncarriers in males of both the GOLDN (P = 0.022) and BPRHS (P = 0.036). Minor allele (T) carriers of rs709592 had significantly higher HOMA-IR (P = 0.032) than C homozygotes in the GOLDN, whereas the T allele carriers of rs709592 tended to have higher HOMA-IR (P = 0.08) than C homozygotes in the BPRHS. In the GOLDN, there was an interaction between rs709592 and dietary carbohydrate on HOMA-IR (P = 0.049). Subjects carrying the T allele of rs709592 had higher HOMA-IR compared only with noncarriers with low carbohydrate intake (≤49.1% energy; P = 0.004). SNPs rs4065321 and rs709592 both significantly interacted with dietary MUFAs and carbohydrate on glucose concentrations in the GOLDN. Our study suggests that PSMD3 variants are associated with insulin resistance in populations of different ancestries and that these relationships may also be modified by dietary factors.