Protein Phosphatase PP1 Negatively Regulates IRF3 in Response to GCRV Infection in Grass Carp ( Ctenopharyngodon idella )

Protein phosphatase-1 (PP1) has an important role in many cell functions, such as cell differentiation, development, immune response and tumorigenesis. However, the specific role of PP1 in the antiviral response in fish remains to be elucidated. In this study, the homolog was identified in the grass...

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Published inFrontiers in immunology Vol. 11; p. 609890
Main Authors Hu, Xudong, Wang, Bing, Feng, Haohao, Zhou, Man, Lin, Yusheng, Cao, Hong
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 22.01.2021
Subjects
antiviral
GCRV
grass carp
Immunology
intestine
PPP1R3G
PPP1R3G
GCRV
intestine
grass carp
antiviral
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Summary:Protein phosphatase-1 (PP1) has an important role in many cell functions, such as cell differentiation, development, immune response and tumorigenesis. However, the specific role of PP1 in the antiviral response in fish remains to be elucidated. In this study, the homolog was identified in the grass carp ( ) and its role in defence against the GCRV infection was investigated. Phylogenetic analysis demonstrated that CiPPP1R3G clustered with homologues from other teleosts. Temporal expression analysis revealed that the expression level of was significantly up-regulated in response to GCRV infection in grass carps, especially in the intestine and head-kidney. Cellular distribution analysis revealed that CiPPP1R3G was located in the nucleus and cytoplasm. Overexpression of significantly negatively regulated the expression of CiIRF3, thus inhibiting its activation. In summary, we systematically analyzed the gene in grass carp and illustrated its function as a negative regulator in the anti-GCRV immune responses.
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This article was submitted to Comparative Immunology, a section of the journal Frontiers in Immunology
Reviewed by: Javier Santander, Memorial University of Newfoundland, Canada; Liu Zhen, Changsha University, China
Edited by: Wei-Dan Jiang, Sichuan Agricultural University, China
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2020.609890