Genetic mapping revealed that the Pun2 gene in Capsicum chacoense encodes a putative aminotransferase
Several genes regulating capsaicinoid biosynthesis including (also known as ), , , and have been studied. However, the gene encoded by in the non-pungent is unknown. This study aimed to identify the gene by genetic mapping using interspecific ( × ) and intraspecific ( × ) populations. QTL mapping us...
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Published in | Frontiers in plant science Vol. 13; p. 1039393 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
01.11.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Several genes regulating capsaicinoid biosynthesis including
(also known as
),
,
, and
have been studied. However, the gene encoded by
in the non-pungent
is unknown. This study aimed to identify the
gene by genetic mapping using interspecific (
×
) and intraspecific (
×
) populations. QTL mapping using the interspecific F
population revealed two major QTLs on chromosomes 3 and 9. Two bin markers within the QTL regions on two chromosomes were highly correlated with the capsaicinoid content in the interspecific population. The major QTL,
on chromosome 3, contained the
gene, indicating that the non-pungency of
may be attributed to a mutation in the
gene. Sequence analysis revealed a 7 bp nucleotide insertion in the 8
exon of
of the non-pungent
. This mutation resulted in the generation of an early stop codon, resulting in a truncated mutant lacking the PLP binding site, which is critical for pAMT enzymatic activity. This insertion co-segregated with the pungency phenotype in the intraspecific F
population. We named this novel
allele
. Taken together, these data indicate that the non-pungency of
is due to the non-functional
allele, and
encodes the
gene. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Zhangsheng Zhu, South China Agricultural University, China; Neftali Ochoa-Alejo, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico; Zhaoming Qi, Northeast Agricultural University, China Edited by: Congli Wang, Northeast Institute of Geography and Agroecology, (CAS), China This article was submitted to Plant Breeding, a section of the journal Frontiers in Plant Science |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2022.1039393 |