The Development, Optimization and Future of Prime Editing

Prime editing is a rapidly developing method of CRISPR/Cas-based genome editing. The increasing number of novel PE applications and improved versions demands constant analysis and evaluation. The present review covers the mechanism of prime editing, the optimization of the method and the possible ne...

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Bibliographic Details
Published inInternational journal of molecular sciences Vol. 24; no. 23; p. 17045
Main Authors Petrova, Irina O, Smirnikhina, Svetlana A
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.12.2023
Subjects
Analysis
Binding Sites
CRISPR
CRISPR-Cas Systems - genetics
Cyclin-dependent kinases
Deoxyribonuclease I
DNA Mismatch Repair
DNA polymerases
Efficiency
Gene Editing
Genetic transcription
Genome editing
Genomes
Genomics
Kinases
Mutation
Optimization
prime editing
Proteins
RNA
RNA polymerase
RNA, Guide, CRISPR-Cas Systems
serine integrase
twinPE
viral delivery
Canada
viral delivery
prime editing
serine integrase
twinPE
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Summary:Prime editing is a rapidly developing method of CRISPR/Cas-based genome editing. The increasing number of novel PE applications and improved versions demands constant analysis and evaluation. The present review covers the mechanism of prime editing, the optimization of the method and the possible next step in the evolution of CRISPR/Cas9-associated genome editing. The basic components of a prime editing system are a prime editor fusion protein, consisting of nickase and reverse transcriptase, and prime editing guide RNA, consisting of a protospacer, scaffold, primer binding site and reverse transcription template. Some prime editing systems include other parts, such as additional RNA molecules. All of these components were optimized to achieve better efficiency for different target organisms and/or compactization for viral delivery. Insights into prime editing mechanisms allowed us to increase the efficiency by recruiting mismatch repair inhibitors. However, the next step in prime editing evolution requires the incorporation of new mechanisms. Prime editors combined with integrases allow us to combine the precision of prime editing with the target insertion of large, several-kilobase-long DNA fragments.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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content type line 23
ObjectType-Review-1
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242317045