Lipid peroxidation, cyclooxygenase enzyme and tumor cell proliferation inhibitory compounds in Cornus kousa fruits

• Published in: Phytomedicine (Stuttgart) Vol. 14; no. 10; pp. 706 - 709
• Main Authors: Vareed, Shaiju K., Schutzki, Robert E., Nair, Muraleedharan G.
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.10.2007; Urban & Fischer Verlag
• Subjects: Anthocyanin; Anthocyanins; Antimitotic agents; Antineoplastic agents; Antineoplastic Agents, Phytogenic - administration & dosage; Antineoplastic Agents, Phytogenic - pharmacology; Antineoplastic Agents, Phytogenic - therapeutic use; Bioflavonoids; Cell Line, Tumor - drug effects; Cell Line, Tumor - enzymology; Cell Proliferation - drug effects; Chemical properties; Cornus; Cyclooxygenase Inhibitors - administration & dosage; Cyclooxygenase Inhibitors - pharmacology; Cyclooxygenase Inhibitors - therapeutic use; Cyclooxygenases; Dogwood; Dose-Response Relationship, Drug; Enzyme inhibitors; Flavones; Flavonoids; Flowering dogwood; Fruit; Health aspects; Humans; Lipid peroxidation; Lipid Peroxidation - drug effects; Nutritional aspects; Phytotherapy; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Polyphenols; Prevention; Prostaglandin-Endoperoxide Synthases - metabolism; United States; Lipid peroxidation; Polyphenols; Anthocyanins; Flavonoids
• ISSN: 0944-7113; 1618-095X
• DOI: 10.1016/j.phymed.2006.09.006

The genus Cornus is well known for its medicinal properties. Bioassay-guided isolation and characterization of C. kousa fruits afforded kaempferol 3- O-rhamnoside (1), myricetin 3- O-rhamnoside (2), kaempferol 3- O-glucoside (3), cornin (4) and stenophyllin (5) in addition to ursolic acid and β-sitosterol. These compounds are isolated for the first time from C. kousa. Compounds 1–5 inhibited Fe 2+ catalyzed lipid peroxidation by 63%, 57%, 61%, 53%, and 51%, at 23, 22, 23, 129, and 108 μM, respectively. Similarly, they inhibited COX-1 and -2 enzymes activities by 24% and 47%, 40% and 37%, 20% and 37%, 52% and 63%, and 48% and 55% respectively, at 231, 215, 226, 258, and 217 μM, respectively. At 129 μM, compound 4 displayed growth inhibition of HCT-116 (colon), MCF-7 (breast), NCI-H460 (lung), SF-268 (central nervous system CNS), and AGS (stomach) human tumor cell lines by 31%, 29%, 40%, 9%, and 28%, respectively. Similarly, compound 5 inhibited the growth of colon, breast, lung, CNS, and stomach tumor cell lines by 0%, 27%, 35%, 16%, and 27%, respectively, at 108 μM.