An innovative technique for inoculating recombinant baculovirus into the silkworm Bombyx mori using lipofectin

• Published in: Research in microbiology Vol. 155; no. 6; pp. 462 - 466
• Main Authors: Wu, Xiaofeng, Cao, Cuiping, Kumar, V.Shyam, Cui, Weizheng
• Format: Journal Article
• Language: English
• Published: Paris Elsevier SAS 01.07.2004; Elsevier
• Subjects: Animals; Baculoviridae - genetics; Baculoviridae - isolation & purification; Baculovirus; Biological and medical sciences; Bombyx - genetics; Bombyx - metabolism; Bombyx - virology; Bombyx mori; Fundamental and applied biological sciences. Psychology; Gene Transfer Techniques; Genetic Vectors; Inoculation technique; Insect Proteins; Larva - genetics; Larva - metabolism; Larva - virology; Microbiology; Miscellaneous; Phosphatidylethanolamines - metabolism; Recombinant baculoviruses; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Silkworm; Virology; Silkworm; Inoculation technique; Recombinant baculoviruses; Microbiology; Insecta; Bombyx mori; Baculovirus; Virus; Baculoviridae; Arthropoda; Bombycidae; Lepidoptera; Invertebrata
• ISSN: 0923-2508; 1769-7123
• DOI: 10.1016/j.resmic.2004.02.006

The insect baculovirus expression system is one of the most effective eukaryotic expression systems known, and has been widely used to produce numerous recombinant proteins. The current traditional inoculation method consists of injecting recombinant baculovirus directly into insects, thus causing potential contamination to the environment due to virus diffusion during the inoculation process. In the present experiment, we directly introduced baculovirus DNA into silkworms using a cationic lipofectin reagent instead of directly injecting the virus. This new method produced the same infection results as the traditional method. A new safe infection technique without direct use of the virus was thus developed.