Expression of Prostaglandin-Endoperoxide Synthase 1 and Prostaglandin-Endoperoxide Synthase 2 in Human Osteoblasts

• Published in: Biochemical and biophysical research communications Vol. 198; no. 3; pp. 955 - 960
• Main Authors: Debrumfernandes, A.J., Laporte, S., Heroux, M., Lora, M., Patry, C., Menard, H.A., Dumais, R., Leduc, R.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.02.1994; Elsevier
• Subjects: Alkaline Phosphatase - metabolism; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Blotting, Northern; Cells, Cultured; Cyclic AMP - metabolism; Dexamethasone - pharmacology; Dinoprostone - metabolism; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Gene Expression - drug effects; Humans; Interleukin-1 - pharmacology; Isoenzymes - biosynthesis; Kinetics; Osteoblasts - drug effects; Osteoblasts - enzymology; Osteoblasts - metabolism; Osteocalcin - biosynthesis; Oxidoreductases; Prostaglandin-Endoperoxide Synthases - biosynthesis; Recombinant Proteins - pharmacology; RNA, Messenger - analysis; RNA, Messenger - biosynthesis; Tumor Necrosis Factor-alpha - pharmacology; Human; Cell culture; Prostaglandin-endoperoxide synthase; Enzyme; Isozyme; Cytokine; Osteoblast; Stimulation; Oxidoreductases; Gene expression; Glucocorticoid
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1994.1136

A Prostaglandin endoperoxide synthase isoenzyme was recently identified in several cell lines. Osteoblasts possess Prostaglandin endoperoxide synthase activity, but it is not known which isoenzymes are present in these cells. Our objective was to identify these isoenzymes in human osteoblasts. Resting cells in culture did not produce measurable amounts of PGE 2 and did not express Prostaglandin endoperoxide synthase-1 or Prostaglandin endoperoxide synthase-2 mRNAs detectable by Northern blot. Treatment with rhIL1α or rhTNFα induced both the expression of Prostaglandin endoperoxide synthase-2 mRNA and the synthesis of PGE 2, rhIL-1α being more potent on an equimolar basis than rhTNFα. Dexamethasone inhibited the increase in Prostaglandin endoperoxide synthase-2 mRNA and the production of PGE 2 induced by both cytokines. These results suggest that Prostaglandin endoperoxide synthase-2 may be the relevant isoenzyme for prostanoid production in human osteoblasts in culture.