Performance of biological phosphorus removal and characteristics of microbial community in the oxic-settling-anaerobic process by FISH analysis

• Published in: Journal of Zhejiang University. A. Science Vol. 9; no. 7; pp. 1004 - 1010
• Main Authors: Wang, Jian-fang, Zhao, Qing-liang, Jin, Wen-biao, You, Shi-jie, Zhang, Jin-na
• Format: Journal Article
• Language: English
• Published: Hangzhou Zhejiang University Press 01.07.2008; School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China%Department of Urban and Civil Engineering, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen 518055, China
• Subjects: Civil Engineering; Classical and Continuum Physics; Engineering; Industrial Chemistry/Chemical Engineering; Mechanical Engineering; 剩余污泥; 污水处理; 生物除磷; 荧光原位杂交; Biological phosphorus removal; Excess sludge reduction; DAPI (4′,6′-diamidino-2-phenyl indol dihydrochloride); Phosphate accumulating organisms (PAOs); X703.1; DQ89; Fluorescent in situ hybridization (FISH); DAPI(4; Fluorescent in sire hybridization (FISH); 6'-diamidino-2-phenyl indol dihydrochloride
• ISSN: 1673-565X; 1862-1775
• DOI: 10.1631/jzus.a0820064

Performance of biological phosphorus removal in the oxic-settling-anaerobic （OSA） process was investigated. Cell staining and fluorescent in situ hybridization （FISH） were used to analyze characteristics and microbial community of sludge. Experimental results showed that phosphorus removal efficiency was near 60% and the amount of biological phosphorus accumulation in aerobic sludge of the OSA system was up to 26.9 mg/g. Biological phosphorus removal efficiency was partially inhibited by carbon sources in the continuous OSA system. Contrasted to the OSA system, biological phosphorus removal efficiency was enhanced by 14% and the average total phosphorus （TP） contents of aerobic sludge were increased by 0.36 mg/g when sufficient carbon sources were supplied in batch experiments. Staining methods indicated that about 35% of microorganisms had typical characteristics of phosphorus accumulating organisms （PAOs）. FISH analysis demonstrated that PAOMIX-binding bacteria were predominant microbial communities in the OSA system, which accounted for around 28% of total bacteria.