STIM1 regulates calcium signaling in taste bud cells and preference for fat in mice

• Published in: The Journal of clinical investigation Vol. 122; no. 6; pp. 2267 - 2282
• Main Authors: Dramane, Gado, Abdoul-Azize, Souleymane, Hichami, Aziz, Vögtle, Timo, Akpona, Simon, Chouabe, Christophe, Sadou, Hassimi, Nieswandt, Bernhard, Besnard, Philippe, Khan, Naim Akhtar
• Format: Journal Article
• Language: English
• Published: United States American Society for Clinical Investigation 01.06.2012
• Subjects: Animals; Antigens, CD36; Arachidonic Acid; Arachidonic Acid - genetics; Arachidonic Acid - metabolism; Biochemistry, Molecular Biology; Biomedical research; Calcium Channels; Calcium Channels - genetics; Calcium Channels - metabolism; Calcium Signaling; Calcium Signaling - physiology; CD36 Antigens - genetics; CD36 Antigens - metabolism; Cellular signal transduction; Dietary Fats; Dietary Fats - pharmacology; Endoplasmic reticulum; Fatty acids; Food Preferences; Food Preferences - physiology; Genetic aspects; Kinases; Life Sciences; Linoleic Acid; Linoleic Acid - pharmacology; Lipids; Membrane Glycoproteins; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Mice; Mice, Knockout; Properties; Proteins; Serotonin; Serotonin - genetics; Serotonin - metabolism; Stromal Interaction Molecule 1; Taste Buds; Taste Buds - metabolism; Taste Perception; Taste Perception - physiology; France
• ISSN: 0021-9738; 1558-8238
• DOI: 10.1172/JCI59953

Understanding the mechanisms underlying oro-gustatory detection of dietary fat is critical for the prevention and treatment of obesity. The lipid-binding glycoprotein CD36, which is expressed by circumvallate papillae (CVP) of the mouse tongue, has been implicated in oro-gustatory perception of dietary lipids. Here, we demonstrate that stromal interaction molecule 1 (STIM1), a sensor of Ca(2+) depletion in the endoplasmic reticulum, mediates fatty acid-induced Ca(2+) signaling in the mouse tongue and fat preference. We showed that linoleic acid (LA) induced the production of arachidonic acid (AA) and lysophosphatidylcholine (Lyso-PC) by activating multiple phospholipase A2 isoforms via CD36. This activation triggered Ca(2+) influx in CD36-positive taste bud cells (TBCs) purified from mouse CVP. LA also induced the production of Ca(2+) influx factor (CIF). STIM1 was found to regulate LA-induced CIF production and the opening of multiple store-operated Ca(2+) (SOC) channels. Furthermore, CD36-positive TBCs from Stim1-/- mice failed to release serotonin, and Stim1-/- mice lost the spontaneous preference for fat that was observed in wild-type animals. Our results suggest that fatty acid-induced Ca(2+) signaling, regulated by STIM1 via CD36, might be implicated in oro-gustatory perception of dietary lipids and the spontaneous preference for fat.