Characterization of a Natural, Stable, Reversible and Colourful Anthocyanidin Network from Sphagnum Moss Based Mainly on the Yellow Trans -Chalcone and Red Flavylium Cation Forms

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 3; p. 709
• Main Authors: Berland, Helge, Andersen, Øyvind M
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 29.01.2021; MDPI AG
• Subjects: 3-deoxyanthocyanidin; Anthocyanins - chemistry; Anthocyanins - genetics; A–E rings; Chalcone - chemistry; Color; colours; equilibrium network; Isomerism; Kinetics; Magnetic Resonance Spectroscopy; Molecular Structure; Pigmentation - genetics; Sphagnopsida - chemistry; Sphagnopsida - genetics; structure elucidation; trans-chalcone; NMR; 3-deoxyanthocyanidin; A–E rings; equilibrium network; structure elucidation; trans-chalcone; colours
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26030709

Anthocyanins with various functions in nature are one of the most important sources of colours in plants. They are based on anthocyanidins or 3-deoxyanthocyanidins having in common a C15-skeleton and are unique in terms of how each anthocyanidin is involved in a network of equilibria between different forms exhibiting their own properties including colour. Sphagnorubin C ( ) isolated from the cell wall of peat moss ( sp.) was in fairly acidic and neutral dimethyl sulfoxide characterized by nuclear magnetic resonance (NMR) and ultraviolet-visible (UV-vis) absorption techniques. At equilibrium, the network of behaved as a two-component colour system involving the reddish flavylium cationic and the yellow -chalcone forms. The additional D- and E-rings connected to the common C15-skeleton extend the -conjugation within the molecule and provide both bathochromic shifts in the absorption spectra of the various forms as well as a low isomerization barrier between the and chalcone forms. The hemiketal and chalcone forms were thus not observed experimentally by NMR due to their short lives. The stable, reversible network of with good colour contrast between its two components has previously not been reported for other natural anthocyanins and might thus have potential in future photochromic systems. This is the first full structural characterization of any naturally occurring anthocyanin chalcone form.