Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario
Historically, bile in the biliary tract has been considered sterile. Most of the series are based on patients with biliary tract diseases or the bile has been obtained with procedures susceptible to contamination. We evaluated the bile in a heterogeneous cohort of liver donors and recipient patients...
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Published in | Frontiers in surgery Vol. 8; p. 621525 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
16.03.2021
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Subjects | |
Online Access | Get full text |
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Summary: | Historically, bile in the biliary tract has been considered sterile. Most of the series are based on patients with biliary tract diseases or the bile has been obtained with procedures susceptible to contamination.
We evaluated the bile in a heterogeneous cohort of liver donors and recipient patients, with samples obtained in a sterile way, directly from the gallbladder and the common bile duct.
We assessed the bile microbiota in six liver donors and in six liver recipients after whole or split liver procedures in adult or pediatric recipients. Bile samples were studied using PCR sequencing of the 16S ribosomal RNA gene amplification (rDNA).
We demonstrated that the bile is sterile, thereby ruling this out as a source of contamination following transplant. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Visceral Surgery, a section of the journal Frontiers in Surgery Edited by: Gabriel Sandblom, Karolinska Institutet (KI), Sweden Reviewed by: Duilio Pagano, Mediterranean Institute for Transplantation and Highly Specialized Therapies (ISMETT), Italy; Gian Luca Grazi, Regina Elena National Cancer Institute (IRCCS), Italy |
ISSN: | 2296-875X 2296-875X |
DOI: | 10.3389/fsurg.2021.621525 |