Very Early Corona Treatment-Mediated Artificial Incubation of Silkworm Eggs and Germline Transformation of Diapause Silkworm Strains

Diapause is an important biological characteristic for many insect species to adapt to adverse environmental conditions and maintain the continuity of the race. Compared with the traditional hydrochloric acid or/and cold storage treatment methods, the artificial corona incubation technology of silkw...

Full description

Saved in:
Bibliographic Details
Published inFrontiers in bioengineering and biotechnology Vol. 10; p. 843543
Main Authors Zhang, Yu-Li, Huang, Yang, Wang, Ping-Yang, Li, Qiang, Bi, Li-Hui, Zhao, Ai-Chun, Xiang, Zhong-Huai, Long, Ding-Pei
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 11.02.2022
Subjects
artificial incubation
Bioengineering and Biotechnology
Bombyx mori
corona treatment
egg diapause
germline transformation
insect
insect
Bombyx mori
egg diapause
germline transformation
corona treatment
artificial incubation
Online AccessGet full text

Cover

More Information
Summary:Diapause is an important biological characteristic for many insect species to adapt to adverse environmental conditions and maintain the continuity of the race. Compared with the traditional hydrochloric acid or/and cold storage treatment methods, the artificial corona incubation technology of silkworm ( Bombyx mori ) eggs has many advantages including, the absence of pollution, easy operation and safety. However, this technology has not yet been applied in sericulture. In this study, we developed a novel artificial corona instrument to successfully disrupt the diapause of newly laid and refrigerated eggs from various Chinese and Japanese lineage silkworm strains. Subsequently, we invented a very early corona treatment (VECT) strategy to prevent the diapause of newly laid silkworm eggs within 4 h of oviposition. The hatching rates of the larvae were more than 95% in all diapause silkworm strains, which was comparable to the effect of the traditional HCl treatment strategy. In addition, we developed a combination strategy of VECT and pre-blastoderm microinjection and successfully created transgenic silkworms in various diapause strains. The results of the current study can aid in improving the corona artificial incubation technology and promote its application in sericulture.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
This article was submitted to Bionics and Biomimetics, a section of the journal Frontiers in Bioengineering and Biotechnology
These authors have contributed equally to this work
Edited by: Huipeng Pan, South China Agricultural University, China
Reviewed by: Hongxia Hua, Huazhong Agricultural University, China
Yanghu SiMa, Soochow University, China
ISSN:2296-4185
2296-4185
DOI:10.3389/fbioe.2022.843543