Two Novel Coding SNPs of SREBP1c Gene are Associated with Body Weight and Average Daily Gain in Bovine

• Published in: Animal biotechnology Vol. 21; no. 3; pp. 170 - 178
• Main Authors: Huang, Yongzhen, Zhang, Enping, Wang, Jing, Huai, Yongtao, Lan, Xianyong, Ma, Liang, Li, Zhuanjian, Ren, Gang, Chen, Fuying, Lei, Chuzhao, Wang, Juqiang, Chen, Hong
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Group 01.07.2010
• Subjects: Animals; Base Sequence; Body Weight - physiology; Cattle; Cattle - genetics; Cattle - physiology; Chi-Square Distribution; DNA - chemistry; DNA - genetics; Forced PCR-RFLP; Genetic Variation; Genotype; Growth traits; Molecular Sequence Data; Polymerase Chain Reaction - veterinary; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; SNP; SREBP1c gene; Sterol Regulatory Element Binding Protein 1 - genetics; Sterol Regulatory Element Binding Protein 1 - physiology
• ISSN: 1049-5398; 1532-2378
• DOI: 10.1080/10495391003768813

It is known that the SREBP1c gene is an important gene responsible for adipogenesis and regulation of the expression of genes controlling fatty acid biosynthesis. Its expression levels increase in parallel with obesity. Therefore, the present study focused on screening the genetic variation within bovine SREBP1c gene and analyzing its effect on growth traits in 1035 individuals belonging to four Chinese cattle breeds (QC, NY, JX, CH) using PCR-SSCP, DNA sequencing, and forced PCR-RFLP methods. The results revealed two novel mutations: NC_007317: g. 10781 C > A (457aa), 10914 G > A (502aa). Association analysis with growth traits in the Nangyang breed indicated that: The SNPs in the bovine SREBP1c gene had significant effects on body weight and average daily gain at birth, 6 and 12 months old (P < 0.05 or P < 0.01). Therefore, these results suggest that the SREBP1c gene is a strong candidate gene that affects growth traits in cattle.