Characterization of a mcr-1 and CRISPR-Cas System Co-harboring Plasmid in a Carbapenemase-Producing High-Risk ST11 Klebsiella pneumoniae Strain
We set out to study the prevalence of the gene in carbapenemase-producing (CPKP) strains, and to determine whether its presence is associated with a fitness cost. A total of 234 clinical CPKP isolates were collected from a tertiary medical center in Taiwan from January 2018 to January 2019. The and...
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Published in | Frontiers in microbiology Vol. 12; p. 762947 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
27.10.2021
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Subjects | |
Online Access | Get full text |
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Summary: | We set out to study the prevalence of the
gene in carbapenemase-producing
(CPKP) strains, and to determine whether its presence is associated with a fitness cost. A total of 234 clinical CPKP isolates were collected from a tertiary medical center in Taiwan from January 2018 to January 2019. The
and carbapenemase genes were detected by polymerase chain reaction (PCR) followed by Sanger sequencing. The
-positive carbapenemase-producing strain was characterized by whole genome sequencing, a plasmid stability test and a conjugation assay.
growth rate and an
virulence test were compared between the parental
-positive strain and its
plasmid-cured strain. We identified only one
positive strain (KP2509), co-harboring
and
, among 234 (1/234, 0.43%) CPKP strains. KP2509 and its
transconjugant showed moderate colistin resistance (MIC = 8 mg/L). The
is located on a large conjugative plasmid (317 kb), pKP2509-MCR, with three replicons, IncHI, IncFIB, and IncN. Interestingly, a complete Type IV-A3 CRISPR-Cas system was identified in pKP2509-MCR. Plasmid pKP2509-MCR was highly stable in KP2509 after 270 generation of passage, and the pKP2509-MCR cured strain PC-KP2509 showed similar growth rate and
virulence in comparison to KP2509. The prevalence of
in CPKP strains remains low in our center. Notably, we identified a large plasmid with multiple replicons containing both the
and the Type IV-3A CRISPR-Cas genes. The further spread of this highly stable plasmid raises concern that it may promote the increase of
prevalence in CPKP. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Jens Andre Hammerl, Bundesinstitut für Risikobewertung, Germany; Jian-Hua Liu, South China Agricultural University, China; Chengming Wang, Auburn University, United States These authors have contributed equally to this work Edited by: Miklos Fuzi, Semmelweis University, Hungary This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2021.762947 |