Inhibitory Effect of Standardized Curcuma xanthorrhiza Supercritical Extract on LPS-Induced Periodontitis in Rats

• Published in: Journal of microbiology and biotechnology Vol. 28; no. 10; pp. 1614 - 1625
• Main Authors: Kook, Kyo Eun, Kim, Changhee, Kang, Wonku, Hwang, Jae-Kwan
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 28.10.2018
• Subjects: Alveolar Bone Loss - pathology; Alveolar Bone Loss - prevention & control; Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Bone Remodeling - drug effects; Curcuma - chemistry; Disease Models, Animal; Gene Expression - drug effects; Gingiva - drug effects; Gingiva - pathology; Inflammation - genetics; Lipopolysaccharides - toxicity; Male; Osteoblasts - cytology; Osteoblasts - drug effects; Osteogenesis - drug effects; Periodontitis - chemically induced; Periodontitis - pathology; Periodontitis - prevention & control; Phenols - standards; Plant Extracts - chemistry; Plant Extracts - pharmacology; Rats; Rats, Sprague-Dawley; 생물학; periodontal inflammation; osteoclastogenesis; Curcuma xanthorrhiza; osteoblastogenesis; periodontitis
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1808.08052

Periodontitis, which is a severe inflammatory disease caused by endotoxins secreted from oral pathogens, destructs gingival tissue and alveolar bone. , commonly called Java turmeric, has been shown to possess anti-bacterial and anti-inflammatory activities. The present study evaluated the inhibitory effect of supercritical extract (CXS) standardized with xanthorrhizol on lipopolysaccharide (LPS)-induced periodontitis in an animal model. LPS was topically injected into the periodontium of Sprague-Dawley rats to induce periodontitis and CXS (30 and 100 mg·kg ·day ) was orally administered after day 12. Histologically, CXS inhibited the collapse of gingival tissue by preventing cell infiltration. CXS significantly downregulated the expression of matrix metalloproteases (MMPs) and inflammation-related biomarkers, such as nuclear factor-kappa B (NF-κB) and interleukin-1 beta (IL-1β) in gingival tissue. CXS also improved bone remodeling by downregulating osteoclastic transcription factors, such as nuclear factor of activated T-cells c1 (NFATc1), tartrate-resistant acid phosphatase (TRAP), and cathepsin K. In addition, CXS upregulated osteoblast differentiation-related markers, alkaline phosphate (ALP) and collagen type I alpha (COLA1). Thus, CXS can ameliorate periodontitis by inhibiting inflammation and improving bone remodeling.