Proteome analysis reveals protein candidates involved in early stages of brain regeneration of teleost fish

• Published in: Neuroscience Vol. 219; pp. 302 - 313
• Main Authors: Ilieş, I, Zupanc, M.M, Zupanc, G.K.H
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 06.09.2012; Elsevier
• Subjects: Animals; Apteronotus leptorhynchus; Biological and medical sciences; Cerebellum - physiology; Electrophoresis, Gel, Two-Dimensional; Fundamental and applied biological sciences. Psychology; Gymnotiformes - physiology; injury; Nerve Regeneration - physiology; Neurology; Proteome; proteomics; regeneration; Vertebrates: nervous system and sense organs; wound healing; IPG; DIGE; regeneration; MHC; 2D-PAGE; major histocompatibility complex; MS/MS; mass spectroscopy; tandem mass spectrometry; immobilized pH gradient; injury; DIA; biological variance analysis; differential in-gel analysis; wound healing; SDS; MALDI-TOF; proteomics; matrix assisted LASER desorption/ionization time-of-flight; MS; PMF; Apteronotus leptorhynchus; BVA; difference gel electrophoresis; two-dimensional polyacrylamide gel electrophoresis; peptide mass fingerprinting; sodium dodecylsulphate; Regeneration; Central nervous system; Proteomics; Early stage; Lesion; Apteroriotus leptorhynchus; Protein; Encephalon
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2012.05.028

Highlights ► We combine a cerebellar lesion paradigm with differential proteome analysis. ► We screen for proteins involved in early stages of regeneration of the cerebellum in a teleost fish. ► We characterize the changes in protein abundance of nearly 800 proteins. ► We identify 11 proteins and discuss their putative function. ► We show that the onset of regeneration occurs within minutes in a regeneration-competent organism.