Differential gene expression profiling in blood from patients with digestive system cancers

• Published in: Biochemical and biophysical research communications Vol. 400; no. 1; pp. 7 - 15
• Main Authors: Honda, Masao, Sakai, Yoshio, Yamashita, Taro, Yamashita, Tatsuya, Sakai, Akito, Mizukoshi, Eishiro, Nakamoto, Yasunari, Tatsumi, Isamu, Miyazaki, Yoshitaka, Tanno, Hiroshi, Kaneko, Shuichi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2010
• Subjects: Aged; Cluster Analysis; Diagnosis; Digestive system cancer; Digestive System Neoplasms - blood; Digestive System Neoplasms - diagnosis; Digestive System Neoplasms - genetics; Female; Gene expression; Gene Expression Profiling - methods; Humans; Male; Middle Aged; Oligonucleotide Array Sequence Analysis; Pathway; Peripheral blood; Pathway; ROC; HCC; SVM; Gene expression; PBMC; AUC; CA 19-9; IFN; NPV; PPV; Peripheral blood; HSC; Diagnosis; Digestive system cancer; CEA; BMI
• ISSN: 0006-291X; 1090-2104; 1090-2104
• DOI: 10.1016/j.bbrc.2010.07.123

► We developed a non-invasive and sensitive diagnostic test for cancer using peripheral blood. ► The expression of blood from digestive systems cancers was different from normal subjects. ► The 25 cancer-differentiating genes were identified. ► The sensitivity and specificity was 100% in training cohort and 87% in validation cohort. To develop a non-invasive and sensitive diagnostic test for cancer using peripheral blood, we evaluated gene expression profiling of blood obtained from patients with cancer of the digestive system and normal subjects. The expression profiles of blood-derived total RNA obtained from 39 cancer patients (11 colon cancer, 14 gastric cancer, and 14 pancreatic cancer) was clearly different from those obtained from 15 normal subjects. By comparing the gene expression profiles of cancer patients and normal subjects, 25 cancer-differentiating genes ( p < 5.0 × 10 −6 and fold differences >3) were identified and an “expression index” deduced from the expression values of these genes differentiated the validation cohort (11 colon cancer, 8 gastric cancer, 18 pancreatic cancer, and 15 normal subjects) into cancer patients and normal subjects with 100% (37/37) and 87% (13/15) accuracy, respectively. Although, the expression profiles were not clearly different between the cancer patients, some characteristic genes were identified according to the stage and species of the cancer. Interestingly, many immune-related genes such as antigen presenting, cell cycle accelerating, and apoptosis- and stress-inducing genes were up-regulated in cancer patients, reflecting the active turnover of immune regulatory cells in cancer patients. These results showed the potential relevance of peripheral blood gene expression profiling for the development of new diagnostic examination tools for cancer patients.