Evaluation of MALDI-TOF Mass Spectrometry in Diagnostic and Environmental Surveillance of Legionella Species: A Comparison With Culture and Mip -Gene Sequencing Technique
spp. are widespread bacteria in aquatic environments with a growing impact on human health. Between the 61 species, is the most prevalent in human diseases; on the contrary, non- species are less detected in clinical diagnosis or during environmental surveillance due to their slow growth in culture...
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Published in | Frontiers in microbiology Vol. 11; p. 589369 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
15.12.2020
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Subjects | |
Online Access | Get full text |
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Summary: | spp. are widespread bacteria in aquatic environments with a growing impact on human health. Between the 61 species,
is the most prevalent in human diseases; on the contrary,
non-
species are less detected in clinical diagnosis or during environmental surveillance due to their slow growth in culture and the absence of specific and rapid diagnostic/analytical tools. Reliable and rapid isolate identification is essential to estimate the source of infection, to undertake containment measures, and to determine clinical treatment. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS), since its introduction into the routine diagnostics of laboratories, represents a widely accepted method for the identification of different bacteria species, described in a few studies on the
clinical and environmental surveillance. The focus of this study was the improvement of MALDI-TOF MS on
non-
species collected during
nosocomial and community surveillance. Comparative analysis with cultural and
-gene sequencing results was performed. Moreover, a phylogenetic analysis was carried out to estimate the correlations amongst isolates. MALDI-TOF MS achieved correct species-level identification for 45.0% of the isolates belonging to the
,
,
, and
species, displaying a high concordance with the
gene sequencing results. In contrast, less reliable identification was found for the remaining 55.0% of the isolates, corresponding to the samples belonging to species not yet included in the database. The phylogenetic analysis showed relevant differences inside the species, regruped in three main clades; among the
clade, a subclade with a divergence of 3.3% from the main clade was observed. Moreover, one isolate, identified as
, displayed a divergence of 3.8% from the corresponding reference strain. However, these findings require supplementary investigation. The results encourage the implementation of MALDI-TOF MS in routine diagnostics and environmental
surveillance, as it displays a reliable and faster identification at the species level, as well as the potential to identify species that are not yet included in the database. Moreover, phylogenetic analysis is a relevant approach to correlate the isolates and to track their spread, especially in unconventional reservoirs, where
prevention is still underestimated. |
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Bibliography: | Edited by: Axel Cloeckaert, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), France This article was submitted to Infectious Diseases, a section of the journal Frontiers in Microbiology Reviewed by: Jaroslav Hrabak, Charles University, Czechia; Maurizio Sanguinetti, Catholic University of the Sacred Heart, Italy; Victoria Girard, Biomerieux, France |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2020.589369 |