Presence of Anti-MDA5 Antibody and Its Value for the Clinical Assessment in Patients With COVID-19: A Retrospective Cohort Study

• Published in: Frontiers in immunology Vol. 12; p. 791348
• Main Authors: Wang, Geng, Wang, Qian, Wang, Yeming, Liu, Changzheng, Wang, Linghang, Chen, Hong, Jiao, Tao, Hu, Chaojun, Lei, Xiaobo, Guo, Li, Ren, Lili, Li, Mengtao, Zhao, Yan, Zeng, Xiaofeng, Zhang, Dingyu, Cao, Bin, Wang, Jianwei
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 20.12.2021
• Subjects: acute respiratory distress syndrome (ARDS); Adult; Aged; anti-melanoma differentiation-associated gene 5 (MDA5) antibody; Antibodies - blood; Antibodies - immunology; autoimmune; COVID-19; COVID-19 - epidemiology; COVID-19 - immunology; COVID-19 - virology; dermatomyositis; Disease Progression; Enzyme-Linked Immunosorbent Assay - methods; Female; Humans; Immunology; innate immunity; Interferon-Induced Helicase, IFIH1 - immunology; Male; Middle Aged; Pandemics; Prognosis; Retrospective Studies; SARS-CoV-2 - physiology; Severity of Illness Index; COVID-19; dermatomyositis; autoimmune; innate immunity; anti-melanoma differentiation-associated gene 5 (MDA5) antibody; acute respiratory distress syndrome (ARDS)
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2021.791348

Striking similarities have been found between coronavirus disease 2019 (COVID-19) and anti-melanoma differentiation-associated gene 5 (MDA5) antibody (Ab)-related dermatomyositis, implying a shared autoinflammatory aberrance. Herein, we aim to investigate whether the anti-MDA5 Ab is present in COVID-19 and correlates with the severity and adverse outcome of COVID-19 patients. We retrospectively recruited 274 adult inpatients with COVID-19 in this study, including 48, 164, and 62 cases of deaths, severe, and non-severe patients respectively. The anti-MDA5 Ab was determined by ELISA and verified by Western Blotting, which indicated that the positive rate of anti-MDA5 Ab in COVID-19 patients was 48.2% (132/274). The clinical and laboratory features, as well as outcomes between patients with positive and negative anti-MDA5 Ab were compared and we found that the anti-MDA5 Ab positive patients tended to represent severe disease (88.6% 66.9%, <0.0001). We also demonstrated that the titer of anti-MDA5 Ab was significantly elevated in the non-survivals (5.95 ± 5.16 8.22 ± 6.64, =0.030) and the positive rate was also higher than that in the survivals (23.5% 12.0%, =0.012). Regarding severe COVID-19 patients, we found that high titer of anti-MDA5 Ab (≥10.0 U/mL) was more prevalent in the non-survivals (31.2% 14.0%, =0.006). Moreover, a dynamic analysis of anti-MDA5 Ab was conducted at different time-points of COVID-19, which revealed that early profiling of anti-MDA5 Ab could distinguish severe patients from those with non-severe ones. Anti-MDA5 Ab was prevalent in the COVID-19 patients and high titer of this antibody is correlated with severe disease and unfavorable outcomes.