Serotyping scheme for Campylobacter jejuni and Campylobacter coli based on direct agglutination of heat-stable antigens

• Published in: Journal of clinical microbiology Vol. 36; no. 2; pp. 335 - 339
• Main Authors: FROST, J. A, OZA, A. N, THWAITES, R. T, ROWE, B
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.02.1998
• Subjects: Agglutination Tests - methods; Animals; Antibodies, Bacterial - analysis; Antigens, Bacterial - immunology; Bacteriology; Biological and medical sciences; Campylobacter coli - classification; Campylobacter coli - immunology; Campylobacter Infections - epidemiology; Campylobacter Infections - immunology; Campylobacter jejuni - classification; Campylobacter jejuni - immunology; England - epidemiology; Epidemiology; Fundamental and applied biological sciences. Psychology; Microbiology; Rabbits; Seroepidemiologic Studies; Serotyping; Wales - epidemiology; United Kingdom; Europe; England; Wales; Human; Prevalence; Typing; Campylobacteraceae; Campylobacter coli; Epidemiology; Campylobacter jejuni; Infection; Antigen; Campylobacter infection; Bacteriosis; Digestive diseases; Bacteria; Agglutination test; Serotype
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/JCM.36.2.335-339.1998

Campylobacter is now the most frequently reported cause of gastrointestinal disease in England and Wales, yet few isolates are characterized beyond the genus level. The majority of isolates are Campylobacter jejuni (90%), with most of the remainder being Campylobacter coli. We describe an adaptation of the Penner serotyping scheme in which passive hemagglutination has been replaced by detection of heat-stable antigens by direct bacterial agglutination; absorbed antisera are used where appropriate. This scheme has been used to type 2,407 C. jejuni samples and 182 C. coli samples isolated in Wales between April 1996 and March 1997. Forty-seven C. jejuni serotypes were identified, with the 10 most prevalent serotypes accounting for 53% of the isolates tested; 19% of the isolates were untypeable. Only fifteen C. coli serotypes were identified, with three serotypes accounting for 69% of the isolates. This scheme provides a baseline for epidemiological studies of C. jejuni and C. coli.