Natural Killer Anti-Tumor Activity Can Be Achieved by In Vitro Incubation With Heat-Killed BCG

• Published in: Frontiers in immunology Vol. 12; p. 622995
• Main Authors: Esteso, Gloria, Aguiló, Nacho, Julián, Esther, Ashiru, Omodele, Ho, Mei M, Martín, Carlos, Valés-Gómez, Mar
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 23.02.2021
• Subjects: BCG strains; bladder cancer; cancer immunology; CD56bright; Immunology; mycobacterial fractions; NK activation; NK activation; CD56bright; mycobacterial fractions; BCG strains; bladder cancer; cancer immunology
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2021.622995

Natural Killer cell receptors allow this heterogeneous immune population to efficiently fight both tumors and infection, so their use as immunotherapy agents is an active field of research. Cytokine activation, particularly by myeloid cell-derived IL15, can induce potent NK anti-tumor responses. While studying the mechanism of action of intravesical instillations of (BCG) as therapy for patients with high risk non-muscle invasive bladder cancer, we showed that BCG can activate a cytotoxic CD56 NK cell population which efficiently recognized bladder cancer cells. This pioneer immunotherapy provides an invaluable model to understand the role of different immune populations in tumor elimination. However, during the propagation of BCG worldwide a large number of genetically diverse BCG substrains developed. Here, we investigated the capacity of different BCG substrains to promote NK cell activation and confirmed that they were able to activate lymphocytes. Tice, Connaught and Moreau were the substrains with a stronger NK activation effect as measured by CD56 upregulation. Surprisingly, dead mycobacteria also stimulated PBMC cultures and we further demonstrate here that subcellular fractions of BCG-Tice, in the absence of live mycobacteria, could also induce an NK cell response. Lipids from BCG-Tice, but not from , stimulated NK cell activation and degranulation, whereas the aqueous fraction of either bacteria did not activate lymphocytes. However, delipidated BCG-Tice bacteria were able to activate effector cells (CD3 CD56 and NK, CD3 CD56 ). These data demonstrate that different components of mycobacteria can stimulate different immune subpopulations resulting in phenotypes suitable for cancer elimination.