Comprehensive Analysis to Identify SPP1 as a Prognostic Biomarker in Cervical Cancer

• Published in: Frontiers in genetics Vol. 12; p. 732822
• Main Authors: Zhao, Kaidi, Ma, Zhou, Zhang, Wei
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 04.01.2022
• Subjects: biomarker; cervical cancer; Genetics; immune infiltration; prognosis; SPP1; SPP1; immune infiltration; biomarker; cervical cancer; prognosis
• ISSN: 1664-8021; 1664-8021
• DOI: 10.3389/fgene.2021.732822

, secreted phosphoprotein 1, is a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Previous studies have proven overexpressed in a variety of cancers and can be identified as a prognostic factor, while no study has explored the function and carcinogenic mechanism of in cervical cancer. We aimed to demonstrate the relationship between expression and pan-cancer using The Cancer Genome Atlas (TCGA) database. Next, we validated expression of cervical cancer in the Gene Expression Omnibus (GEO) database, including GSE7803, GSE63514, and GSE9750. The receiver operating characteristic (ROC) curve was used to evaluate the feasibility of as a differentiating factor by the area under curve (AUC) score. Cox regression and logistic regression were performed to evaluate factors associated with prognosis. The -binding protein network was built by the STRING tool. Enrichment analysis by the R package clusterProfiler was used to explore potential function of . The single-sample GSEA (ssGSEA) method from the R package GSVA and TIMER database were used to investigate the association between the immune infiltration level and expression in cervical cancer. Pan-cancer data analysis showed that expression was higher in most cancer types, including cervical cancer, and we got the same result in the GEO database. The ROC curve suggested that could be a potential diagnostic biomarker (AUC = 0.877). High expression was associated with poorer overall survival (OS) ( = 0.032). Further enrichment and immune infiltration analysis revealed that high expression was correlated with regulating the infiltration level of neutrophil cells and some immune cell types, including macrophage and DC. expression was higher in cervical cancer tissues than in normal cervical epithelial tissues. It was significantly associated with poor prognosis and immune cell infiltration. Thus, may become a promising prognostic biomarker for cervical cancer patients.